In silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni.

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dc.contributor.authorCosta, Marcela Pereira
dc.contributor.authorOliveira, Victor Fernandes de
dc.contributor.authorPereira, Roberta Verciano
dc.contributor.authorAbreu, Fabiano Carlos Pinto de
dc.contributor.authorPassos, Liana Konovaloff Jannotti
dc.contributor.authorBorges, William de Castro
dc.contributor.authorCota, Renata Guerra de Sá
dc.date.accessioned2017-10-18T13:38:55Z
dc.date.available2017-10-18T13:38:55Z
dc.date.issued2015
dc.description.abstractUbiquitin-conjugating enzymes (Ub-E2) perform the second step of ubiquitination and, consequently, are essential for regulating proteolysis and for modulating protein function, interactions and trafficking. Previously, our group demonstrated the crucial role of ubiquitination and the Ubproteasome pathway during the Schistosoma mansoni life cycle. In the present investigation, we used a homology-based genome-wide bioinformatics approach to identify and molecularly characterise the Ub-E2 enzymes in S. mansoni. The putative functions were further investigated through molecular phylogenetic and expression profile analyses using cercariae, adult worms, eggs and mechanically transformed schistosomula (MTS) cultured in vitro for 3.5 h or 1 or 3 days. We identified, via in silico analysis, 17 Ub-E2 enzymes with conserved structural characteristics: the beta-sheet and the helix-2 form a central core bordered by helix-1 at one side and helix-3 and helix-4 at the other. The observed quantitative differences in the steady-state transcript levels between the cercariae and adult worms may contribute to the differential protein ubiquitination observed during the parasite’s life cycle. This study is the first to identify and characterise the E2 ubiquitin conjugation family in S. mansoni and provides fundamental information regarding their molecular phylogenetics and developmental expression during intra-mammalian stages.pt_BR
dc.identifier.citationCOSTA, M. P. et al. In silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni. Parasitology Research, v. 14, n. 5, p. 1769-1777, maio 2015. Disponível em: <https://link.springer.com/article/10.1007%2Fs00436-015-4362-x>. Acesso em: 16 jun. 2017.pt_BR
dc.identifier.doihttps://doi.org/10.1007/s00436-015-4362-x
dc.identifier.issn1432-1955
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/8969
dc.identifier.uri2https://link.springer.com/article/10.1007%2Fs00436-015-4362-xpt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectUbiquitinationpt_BR
dc.subjectDifferential expressionpt_BR
dc.titleIn silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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