In silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni.
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Data
2015
Autores
Costa, Marcela Pereira
Oliveira, Victor Fernandes de
Pereira, Roberta Verciano
Abreu, Fabiano Carlos Pinto de
Passos, Liana Konovaloff Jannotti
Borges, William de Castro
Cota, Renata Guerra de Sá
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Resumo
Ubiquitin-conjugating enzymes (Ub-E2) perform
the second step of ubiquitination and, consequently, are essential
for regulating proteolysis and for modulating protein function,
interactions and trafficking. Previously, our group demonstrated
the crucial role of ubiquitination and the Ubproteasome
pathway during the Schistosoma mansoni life cycle.
In the present investigation, we used a homology-based
genome-wide bioinformatics approach to identify and molecularly
characterise the Ub-E2 enzymes in S. mansoni. The
putative functions were further investigated through molecular
phylogenetic and expression profile analyses using cercariae,
adult worms, eggs and mechanically transformed
schistosomula (MTS) cultured in vitro for 3.5 h or 1 or 3 days.
We identified, via in silico analysis, 17 Ub-E2 enzymes with
conserved structural characteristics: the beta-sheet and the
helix-2 form a central core bordered by helix-1 at one side
and helix-3 and helix-4 at the other. The observed quantitative
differences in the steady-state transcript levels between the
cercariae and adult worms may contribute to the differential
protein ubiquitination observed during the parasite’s life cycle.
This study is the first to identify and characterise the E2 ubiquitin
conjugation family in S. mansoni and provides fundamental
information regarding their molecular phylogenetics
and developmental expression during intra-mammalian
stages.
Descrição
Palavras-chave
Ubiquitination, Differential expression
Citação
COSTA, M. P. et al. In silico analysis and developmental expression of ubiquitin-conjugating enzymes in Schistosoma mansoni. Parasitology Research, v. 14, n. 5, p. 1769-1777, maio 2015. Disponível em: <https://link.springer.com/article/10.1007%2Fs00436-015-4362-x>. Acesso em: 16 jun. 2017.