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http://www.repositorio.ufop.br/jspui/handle/123456789/8189
Title: | Cytotoxicity, anti-poliovirus activity and in silico biological evaluation of constituents from Maytenus gonoclada (Celastraceae). |
Authors: | Oliveira, Mauro Lúcio Gonçalves de Assenço, Regina Aparecida Gomes Silva, Grácia Divina de Fátima Lopes, Júlio César Dias Silva, Fernando César Lanna, Maria Célia da Silva Magalhães, José Carlos de Duarte, Lucienir Pains Vieira Filho, Sidney Augusto |
Keywords: | VERO cells Cytotoxicity Apoptosis ChemMapper PASS online |
Issue Date: | 2014 |
Citation: | OLIVEIRA, M. L. G. de et al. Cytotoxicity, anti-poliovirus activity and in silico biological evaluation of constituents from Maytenus gonoclada (Celastraceae). International Journal of Pharmacy and Pharmaceutical Sciences, v. 6, p. 130-137, 2014. Disponível em: <https://innovareacademics.in/journals/index.php/ijpps/article/view/2553>. Acesso em: 16 jun. 2017. |
Abstract: | Objective: The in silico free access web tools PASS online and ChemMapper were used to predict potential biological activities of compounds 1 to 8 isolated from Maytenus gonoclada (Celastraceae). The constituents 4’-O-methylepigalocatequin (6), tingenone (7) and proanthocyanidin A (8), and ethanolic extracts were subjected to in vitro cytotoxicity using VERO cells and anti-Poliovirus assays. Methods: QSAR and molecular superposition, correlating the average number of pharmacophores were used in the prediction studies. Cellular line VERO ATCC CCL-81 was used to determine anti-Poliovirus effect, observed by colorimetric (MTT) method. The annexing V/propidium iodide assay was used to determine the occurrence of apoptosis in the cytotoxicity assays. Results: The experimental results found for constituents 6-8 were in accordance with observed data obtained through PASS online and ChemMapper simulation. Conclusion: Compound 7 showed higher cytotoxic and apoptosis induction properties, and 6 and 8 presented anti-Poliovirus activity. |
URI: | http://www.repositorio.ufop.br/handle/123456789/8189 |
metadata.dc.identifier.uri2: | https://innovareacademics.in/journals/index.php/ijpps/article/view/2553 |
ISSN: | 0975-1491 |
Appears in Collections: | DEFAR - Artigos publicados em periódicos |
Files in This Item:
File | Description | Size | Format | |
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ARTIGO_CytotoxicityAntiPoliovirus.pdf Restricted Access | 422 kB | Adobe PDF | View/Open |
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