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http://www.repositorio.ufop.br/jspui/handle/123456789/7399
Title: | LC UV methodology for simultaneous. |
Authors: | Souza, Jacqueline de Kano, Eunice Kazue Koono, Eunice Emiko Mori Schramm, Simone Grigoleto Porta, Valentina Storpirtis, Sílvia |
Keywords: | Column liquid chromatography Lamivudine Zidovudine Stavudine |
Issue Date: | 2008 |
Citation: | SOUZA, J de et al. LC UV methodology for simultaneous. Chromatographia, Wiesbaden, v. 69, p. S231-S235, 2008. Disponível em: <http://link.springer.com/article/10.1365/s10337-008-0913-y> Acesso em: 10 jan. 2017 |
Abstract: | This study describes an accurate, sensitive, and specific chromatographic method for the simultaneous quantitative determination of lamivudine and zidovudine in human blood plasma, using stavudine as an internal standard. The chromatographic separation was performed using a C8 column (150 9 4.6 mm, 5 lm), and ultraviolet absorbency detection at 270 nm with gradient elution. Two mobile phases were used. Phase A contained 10 mM potassium phosphate and 3% acetonitrile, whereas Phase B contained methanol. A linear gradient was used with a variability of A-B phase proportion from 98–2% to 72–28%, respectively. The drug extraction was performed with two 4 mL aliquots of ethyl acetate. |
URI: | http://www.repositorio.ufop.br/handle/123456789/7399 |
metadata.dc.identifier.uri2: | http://link.springer.com/article/10.1365/s10337-008-0913-y |
metadata.dc.identifier.doi: | https://doi.org/10.1365/s10337-008-0913-y |
ISSN: | 0009-5893 |
Appears in Collections: | DEFAR - Artigos publicados em periódicos |
Files in This Item:
File | Description | Size | Format | |
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ARTIGO_LCUVMethodology.pdf Restricted Access | 236,17 kB | Adobe PDF | View/Open |
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