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Título : | Immunoproteomics approach for the discovery of antigens applied to the diagnosis of canine visceral leishmaniasis. |
Autor : | Costa, Scarleth Silva Santos, Lucas Magno Oliveira Freire, Larissa Chaves Tedeschi, Ana Luiza Filizzola Ribeiro, Naianda Rezende Queiroz, Mariana Helena Rodrigues Beraldo Neto, Emídio Pimenta, Daniel Carvalho Galvani, Nathália Coral Luiz, Gabriel Paulino Oliveira, Maria Eduarda de Ávila, Ricardo Andrez Machado de Carvalho, Ana Maria Ravena Severino Brigido, Bryan Victor Serafim Reis, Alexandre Barbosa Fernandes, Ana Paula Salles Moura Coelho, Eduardo Antônio Ferraz Roatt, Bruno Mendes Souza, Daniel Menezes Duarte, Mariana Costa |
Palabras clave : | Leishmaniasis Proteomic Immunoproteomic Serological diagnosis |
Fecha de publicación : | 2023 |
Citación : | COSTA, S. S. et al. Immunoproteomics approach for the discovery of antigens applied to the diagnosis of canine visceral leishmaniasis. Acta Tropica, v. 241, artigo 106865, 2023. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0001706X23000529?via%3Dihub>. Acesso em: 01 ago. 2023. |
Resumen : | In the present study, an immunoproteomic approach using Leishmania infantum parasites isolated from naturally infected dogs from an endemic region of the disease, was carried out to identify new antigens to be used in the diagnosis of canine visceral leishmaniasis (CVL). Protein extracts, obtained from parasites isolated from asymptomatic (CanLA) and symptomatic (CanLS) dogs, were used to perform the two-dimensional gels. Western Blotting assays were carried out by employing a pool of sera from dogs with visceral leishmaniasis (CanLA or CanLS), healthy dogs from an endemic area, or dogs with similar diseases associated with cross-reactions (babesiosis and ehrlichiosis). With these results, it was possible to exclude the spots that showed a crossreactivity of the sera from groups of healthy dogs, and those with babesiosis or ehrlichiosis. Taken together, 20 proteins were identified, 15 of which have already been described in the literature and 5 of which are hypothetical. An immunogenomic screen strategy was applied to identify conserved linear B-cell epitopes in the identified hypothetical proteins. Two peptides were synthesized and tested in ELISA experiments as a proof of concept for the validation of our immunoproteomics findings. The results demonstrated that the antigens presented sensitivity and specificity values ranging from 81.93% to 97.59% and 78.14 to 85.12%, respectively. As a comparative antigen, a preparation of a Leishmania extract showed sensitivity and specificity values of 75.90% and 74.88%, respectively. The present study was able to identify proteins capable of being used for the serodiagnosis of canine visceral leishmaniasis. |
URI : | http://www.repositorio.ufop.br/jspui/handle/123456789/17542 |
metadata.dc.identifier.uri2: | https://www.sciencedirect.com/science/article/pii/S0001706X23000529?via%3Dihub |
metadata.dc.identifier.doi: | https://doi.org/10.1016/j.actatropica.2023.106865 |
ISSN : | 0001-706X |
Aparece en las colecciones: | DEACL - Artigos publicados em periódicos |
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Fichero | Descripción | Tamaño | Formato | |
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ARTIGO_ImmunoproteomicsApproachDiscovery.pdf Restricted Access | 5,94 MB | Adobe PDF | Visualizar/Abrir |
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