Por favor, use este identificador para citar o enlazar este ítem:
http://www.repositorio.ufop.br/jspui/handle/123456789/13833
Título : | Acarbose presents in vitro and in vivo antileishmanial activity against Leishmania infantum and is a promising therapeutic candidate against visceral leishmaniasis. |
Autor : | Costa, Rafaella Rodrigues Silva, João Augusto Oliveira da Reis, Thiago Alves Rosa dos Tavares, Grasiele de Sousa Vieira Mendonça, Débora Vasconcelos Costa Freitas, Camila Simões de Lage, Daniela Pagliara Martins, Vívian Tamietti Antinarelli, Luciana Maria Ribeiro Machado, Amanda Sanchez Bandeira, Raquel Soares Ribeiro, Fernanda Ludolf Santos, Thaís Teodoro de Oliveira Brito, Rory Cristiane Fortes de Humbert, Maria Victoria Souza, Daniel Menezes Duarte, Mariana Costa Chávez Fumagalli, Miguel Angel Roatt, Bruno Mendes Coimbra, Elaine Soares Coelho, Eduardo Antônio Ferraz |
Palabras clave : | Treatment Drug repositioning Miltefosine |
Fecha de publicación : | 2020 |
Citación : | COSTA, R. R. et al. Acarbose presents in vitro and in vivo antileishmanial activity against Leishmania infantum and is a promising therapeutic candidate against visceral leishmaniasis. Medical Microbiology and Immunology, v. 210, p. 133-147, abr. 2021. Disponível em: <https://link.springer.com/article/10.1007%2Fs00430-021-00707-4>. Acesso em: 10 jun. 2021. |
Resumen : | Treatment against visceral leishmaniasis (VL) is mainly hampered by drug toxicity, long treatment regimens and/or high costs. Thus, the identifcation of novel and low-cost antileishmanial agents is urgent. Acarbose (ACA) is a specifc inhibitor of glucosidase-like proteins, which has been used for treating diabetes. In the present study, we show that this molecule also presents in vitro and in vivo specifc antileishmanial activity against Leishmania infantum. Results showed an in vitro direct action against L. infantum promastigotes and amastigotes, and low toxicity to mammalian cells. In addition, in vivo experiments performed using free ACA or incorporated in a Pluronic® F127-based polymeric micelle system called ACA/ Mic proved efective for the treatment of L. infantum-infected BALB/c mice. Treated animals presented signifcant reductions in the parasite load in their spleens, livers, bone marrows and draining lymph nodes when compared to the controls, as well as the development of antileishmanial Th1-type humoral and cellular responses based on high levels of IFN-γ, IL-12, TNF-α, GM-CSF, nitrite and IgG2a isotype antibodies. In addition, ACA or ACA-treated animals sufered from low organ toxicity. Treatment with ACA/Mic outperformed treatments using either Miltefosine or free ACA based on parasitological and immunological evaluations performed one and 15 days post-therapy. In conclusion, data suggest that the ACA/Mic is a potential therapeutic agent against L. infantum and merits further consideration for VL treatment. |
URI : | http://www.repositorio.ufop.br/jspui/handle/123456789/13833 |
metadata.dc.identifier.uri2: | https://link.springer.com/article/10.1007%2Fs00430-021-00707-4 |
metadata.dc.identifier.doi: | https://doi.org/10.1007/s00430-021-00707-4 |
ISSN : | 1432-1831 |
Aparece en las colecciones: | DECBI - Artigos publicados em periódicos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
ARTIGO_AcarbosePresentsVitro.pdf Restricted Access | 1,5 MB | Adobe PDF | Visualizar/Abrir |
Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.