Navegando por Autor "Sousa, Lucas Resende Dutra"
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Item Anti-inflammatory, antimicrobial, antioxidant and photoprotective investigation of red propolis extract as sunscreen formulation in polawax cream.(2023) Valverde, Thalita Marcolan; Soares, Bruna Nayane Gonçalves de Souza; Nascimento, Andréa Mendes do; Andrade, Ângela Leão; Sousa, Lucas Resende Dutra; Vieira, Paula Melo de Abreu; Santos, Vagner Rodrigues; Seibert, Janaína Brandão; Almeida, Tatiane Cristine Silva de; Rodrigues, Caio Fabrini; Oliveira, Samantha Roberta Machado de; Martins, Flaviano dos Santos; Ferreira Júnior, Jeronimo Geraldo; Santos, Viviane Martins Rebello dosMany activities have been described for propolis, including, antiviral, antibacterial, antifungal, anti-inflammatory, immunoregulatory, antioxidant and wound healing properties. Recently, propolis has been highlighted due to its potential application in the pharmaceutical and cosmetic industries, motivating a better understanding of its antioxidant and anti-inflammatory activities. Propolis and its main polyphenolic compounds presented high antioxidant activity, and effectiveness as broad spectrum UVB and UVA photoprotection sunscreens. Through a qualitative phytochemical screening, the ethanolic red propolis extracts (EEPV) (70% at room temperature and 70% at a hot temperature) presented a positive result for flavonoids and terpenoids. It presented an antioxidant activity for reducing 50% of DPPH of 17 and 12 µg/mL for extraction at room temperature and at a hot temperature, respectively. The UPLC-QTOF-MS/MS analysis allowed the annotation of 40 substances for EEPV-Heated and 42 substances for EEPV-Room Temperature. The IC50 results of the ABTS scavenging activity was 4.7 µg/mL for both extractions, at room temperature and at a hot temperature. Additionally, we also evaluated the cytotoxic profile of propolis extracts against macrophage (RAW 264.7 cells) and keratinocytes (HaCaT cells), which showed non-cytotoxic doses in cell viability assays even after a long period of exposure. In addition, propolis extracts showed antibacterial activity for Gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis), demonstrating potential biological activity for the creation of formulations aimed at disease control and prevention.Item Avaliação da atividade antioxidante e fenóis totais dos óleos extraídos das sementes de Moringa oleifera lam.(2020) Anunciação, Kamila de Fátima; Sousa, Lucas Resende Dutra; Amparo, Tatiane Roquete; Souza, Gustavo Henrique Bianco de; Vieira, Paula Melo de Abreu; Santos, Viviane Martins Rebello dos; Melo, Tânia Márcia SacramentoA Moringa oleifera Lamarck é uma planta nativa da Índia, que apresenta em suas sementes e folhas uma variedade de compostos orgânicos reconhecidos pela alta capacidade antioxidante. Este trabalho teve como objetivo avaliar a atividade antioxidante in vitro de extratos e óleos das sementes e sementes/folhas da M. oleífera Lam. Também foi determinado o teor de compostos fenólicos totais dos extratos pelo método de Folin-Ciocalteu e avaliação da citotoxicidade para fibroblastos humanos pelo método de sulforodamina B. Os resultados mostraram que o extrato etanólico da semente apresentou maior atividade antioxidante, o teor de fenólicos totais desse extrato foi de 235,11 ± 73,49 mg EAG/g e não apresentou elevada citotoxicidade nas concentrações testadas. O extrato etanólico de sementes de M. oleífera Lam possui bom potencial antioxidante e baixa citotoxicidade.Item Benznidazole, itraconazole, and their combination for the treatment of chronic experimental Chagas disease in dogs.(2022) Cunha, Eleonora Lima Alves; Torchelsen, Fernanda Karoline Vieira da Silva; Fonseca, Kátia da Silva; Sousa, Lucas Resende Dutra; Vieira, Paula Melo de Abreu; Carneiro, Cláudia Martins; Pinto, Kelerson Mauro de Castro; Torres, Rosália Morais; Lana, Marta deTreatment for Chagas disease has limited efficacy in the chronic phase. We evaluated benznidazole (BZ) and itraconazole (ITZ) individually and in association in dogs 16 months after infection with a BZ-resistant Trypa- nosoma cruzi strain. Four study groups (20 animals) were evaluated and treated for 60 days with BZ, ITZ, or BZ + ITZ, and maintained in parallel to control group infected and not treated (INT). All dogs were evaluated in the first, sixth, 12th, 18th and 24th months of study. Polymerase chain reaction (PCR) was negative in 2 of 3 animals in the BZ + ITZ group, 2 of 5 in the BZ group, and 4 of 5 in the ITZ group. Hemoculture performed in the 24th month was negative in all groups. Enzyme-linked immunoassay remained reactive in all treated animals. Echocardiography differentiated treated animals from control animals. Quantitative PCR analysis of cardiac tissue was negative in the BZ + ITZ and BZ groups, positive in 2 of 5 dogs in the ITZ group and in 2 of 3 dogs in the control group, but negative in colon tissue in all groups. Inflammation was significantly reduced in the right atrium and left ventricle of dogs treated with BZ + ITZ and BZ compared with those receiving ITZ alone. Fibrosis was absent in most dogs treated with BZ + ITZ, mild in those treated with BZ or ITZ alone, and intense in the control group. Parasitological and histopathological evaluations showed that BZ + ITZ treatment improved or stabilized the clinical condition of the dogs.Item Chemical constituents, antioxidant potential, antibacterial study and photoprotective activity of Brazilian corn silk extract.(2022) Azevedo, Amanda Scofield de; Seibert, Janaína Brandão; Amparo, Tatiane Roquete; Antunes, Amanda dos Santos; Sousa, Lucas Resende Dutra; Souza, Gustavo Henrique Bianco de; Teixeira, Luiz Fernando de Medeiros; Vieira, Paula Melo de Abreu; Santos, Viviane Martins Rebello dos; Nascimento, Adriana Mendes do; Nascimento, Andréa Mendes doThe aim of this work was to determine the total phenolic content and evaluate the antioxidant, antibacterial, photoprotective and cytotoxic properties of corn silk ethanol extract prepared from corn silk that was air-dried at room temperature. Corn silk ethanol extract has a high content of phenolic compounds. The concentrations of ethanol extract required to produce 50% of maximal effect (EC50) in DPPH and ABTS radical scavenging assays were 489.0 and 166.1 μg/mL respectively, indicating low activity when compared to the positive control. The ethanolic extract of corn silk showed antibacterial activity, mainly on Gram-positive bacteria. The in vitro sun protection factor (SPF) of the ethanolic extract of corn silk incorporated into sunscreen UVA-UVB 5% gel with Pemulen TR-1® was evaluated for the first time. A relative increase in SPF efficacy was observed for the extract when the sunscreen was incorporated. The ethanolic extract of corn silk and the gel were considered non-cytotoxic. The corn silk ethanolic extract was characterized using ultra performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS). Eleven compounds including flavonoids, coumaric acid derivatives, terpenoid, hydroxycinnamic acid derivative and quinic acid derivative were suggested based on their accurate mass and MS/MS spectra.Item Controlled release and cell viability of ketoconazole incorporated in PEG 4000 derivatives.(2023) Inácio, Carolina Rodrigues; Nascimento, Gabriel S.; Barboza, Ana Paula Moreira; Neves, Bernardo Ruegger Almeida; Andrade, Ângela Leão; Teixeira, Gabriel Marques; Sousa, Lucas Resende Dutra; Vieira, Paula Melo de Abreu; Novack, Kátia Monteiro; Santos, Viviane Martins Rebello dosIn recent years, polymeric materials have been gaining prominence in studies of controlled release systems to obtain improvements in drug administration. These systems present several advantages compared with conventional release systems, such as constant maintenance in the blood concentration of a given drug, greater bioavailability, reduction of adverse effects, and fewer dosages required, thus providing a higher patient compliance to treatment. Given the above, the present work aimed to synthesize polymeric matrices derived from polyethylene glycol (PEG) capable of promoting the controlled release of the drug ketoconazole in order to minimize its adverse effects. PEG 4000 is a widely used polymer due to its excellent properties such as hydrophilicity, biocompatibility, and non-toxic effects. In this work, PEG 4000 and derivatives were incorporated with ketoconazole. The morphology of polymeric films was observed by AFM and showed changes on the film organization after drug incorporation. In SEM, it was possible to notice spheres that formed in some incorporated polymers. The zeta potential of PEG 4000 and its derivatives was determined and suggested that the microparticle surfaces showed a low electrostatic charge. Regarding the controlled release, all the incorporated polymers obtained a controlled release profile at pH 7.3. The release kinetics of ketoconazole in the samples of PEG 4000 and its derivatives followed first order for PEG 4000 HYDR INCORP and Higuchi for the other samples. Cytotoxicity was determined and PEG 4000 and its derivatives were not cytotoxic.Item Evaluation of anti-candida albicans activity and release of ketoconazole in PMMA-G-PEG 4000 films.(2022) Reynaldo, Juliana Ribeiro; Novack, Kátia Monteiro; Sousa, Lucas Resende Dutra; Vieira, Paula Melo de Abreu; Amparo, Tatiane Roquete; Souza, Gustavo Henrique Bianco de; Teixeira, Luiz Fernando de Medeiros; Barboza, Ana Paula Moreira; Neves, Bernardo Ruegger Almeida; Alvarenga, Meiry Edivirges; Martins, Felipe Terra; Santos, Viviane Martins Rebello dosModified release systems depend on the selection of an appropriate agent capable of controlling the release of the drug, sustaining the therapeutic action over time, and/or releasing the drug at the level of a particular tissue or target organ. Polyethylene glycol 4000 (PEG 4000) is commonly employed in drug release formulations while polymethyl methacrylate (PMMA) is non-toxic and has a good solubility in organic solvents. This study aimed at the incorporation of ketoconazole in PMMA-g-PEG 4000 and its derivatives, thus evaluating its release profile and anti-Candida albicans and cytotoxic activities. Ketoconazole was characterized and incorporated into the copolymers. The ketoconazole incorporated in the copolymer and its derivatives showed an immediate release profile. All copolymers with ketoconazole showed activity against Candida albicans and were non-toxic to human cells in the entire concentration tested.Item Green propolis : in vitro photoprotective and photostability studies of single and incorporated extracts in a sunscreen formulation.(2020) Almeida, Wanessa Aparecida da Silveira; Sousa, Lucas Resende Dutra; Antunes, Amanda dos Santos; Azevedo, Amanda Scofield de; Nascimento, Andréa Mendes do; Amparo, Tatiane Roquete; Souza, Gustavo Henrique Bianco de; Santos, Orlando David Henrique dos; Andrade, Ângela Leão; Cazati, Thiago; Vieira, Paula Melo de Abreu; Bueno, Paula Carolina Pires; Santos, Viviane Martins Rebello dosPropolis is a natural, non-toxic resin produced by honey bees that has been used for hundreds of years for its biological activities, such as antimicrobial, anti-inflammatory, anesthetic, cytostatic, and cariostatic properties. Currently, it is used in food, pharmaceutical, and cosmetic industries. The aim of this work was to evaluate the antioxidant activity, sun protection factor, and photostability of different hydroalcoholic extracts of green propolis. All extracts prepared presented high absorption in the UVB region. The extract of 70% green propolis (high temperature) was incorporated into Gel Permulen TR-1. This new formulation presented a higher value of sun protection factor. Besides that, the formulation developed with Gel Permulen TR-1 and the hydroalcoholic extract of 70% green propolis (high temperature) showed good photostability and it was safe to be applied on the skin according the HET-CAM test. These results indicated the potential of hydroalcoholic extracts of 70% green propolis (high temperature) for use in sunscreen.Item In vitro photoprotective evaluation and development of novel nanoemulsion with chromone derivative.(2021) Antunes, Amanda dos Santos; Gouveia, Ana Paula Araújo; Diogo, Gabriela Maciel; Taylor, Jason Guy; Sousa, Lucas Resende Dutra; Amparo, Tatiane Roquete; Perasoli, Fernanda Barçante; Santos, Orlando David Henrique dos; Cazati, Thiago; Vieira, Paula Melo de Abreu; Penido, Ricardo Gonçalves; Santos, Viviane Martins Rebello dosChromone derivatives exhibiting high absorbance values in the UVA/UVB region were synthesized, and their photoprotective properties were evaluated. Chromones were prepared according to known literature procedures and characterized by high resolution mass spectrometry, infrared (IR) and nuclear magnetic resonance (NMR) spectroscopy. The in vitro solar protection factor (SPF) was determined by the Mansur method and cytotoxicity was evaluated using the sulforhodamine B assay. Two of the chromones synthesized demonstrated suitable SPF values and displayed no cytotoxic effect towards MRC-5 human fibroblasts at the tested concentrations, indicating great potential for future in vivo assays and clinical trials. Finally, the lead compound was incorporated into a nanoemulsion. Nanoemulsions showed high droplet size homogeneity and excellent stability. Chromones bearing methoxy substituents were found to be the most promising compounds with ideal photoprotective properties desirable for utilization and incorporation in sunscreen formulations.Item New studies in vitro antibacterial evaluation and cell viability in PMMA-G-PEG 4000 derivatives with encapsulated Erythromycin.(2022) Azevedo, Maria Luíza Schaefer; Rezende, Giselle A. S.; Sousa, Lucas Resende Dutra; Reis, Rúbia Castro Fernandes Melo; Novack, Kátia Monteiro; Barboza, Ana Paula Moreira; Neves, Bernardo Ruegger Almeida; Amparo, Tatiane Roquete; Teixeira, Luiz Fernando de Medeiros; Vieira, Paula Melo de Abreu; Perasoli, Fernanda Barçante; Santos, Viviane Martins Rebello dosSynthetic polymers have been extensively studied in several areas of knowledge, one of which is the pharmaceutical. The drug when encapsultated into the PMMA-g-PEG 4000 matrix can produce fewer side effects. The most frequent side effects of erythromycin are gastrointestinal and are dose related. Nausea, vomiting and diarrhea occur in low frequency with the usual oral doses. The drug used in this study was Erythromycin, an antimicrobial that acts directly on bacterial protein synthesis. Erythromycin was encapsulated into PMMA-g-PEG 4000 derivatives. All copolymers afther encapsultaions were characterized and identified by atomic force microscopy (AFM). In vitro assays of release, antimicrobial activity and cytotoxicity were performed for the copolymers obtained. The copolymers PMMA-g-PEG 4000 HAL and PMMA-g-PEG 4000 ACET showed drug controlled release profiles. All PMMA-g-PEG 4000 derivatives showed antibacterial activity against Staphylococcus aureus and did not show a cytotoxic effect on human fibroblasts.Item Novel PEG 4000 derivatives and its use in controlled release of drug indomethacin.(2020) Nascimento, Lúbia Guaima; Lopes, Suellen A.; Teodolino, Ayron Breno Lima; Novack, Kátia Monteiro; Barboza, Ana Paula Moreira; Neves, Bernardo Ruegger Almeida; Azevedo, Maria Luíza Schaefer; Sousa, Lucas Resende Dutra; Santos, Viviane Martins Rebello dosThe insertion of functional groups in polymer compounds may facilitate their interaction with different drugs. PEG polymers are widely used for their low melting point, low toxicity, drug compatibility, and hydrophilicity. They are used as pharmaceutical excipients for the formulation of conventional or modified released drugs and are designed to be upgraded as drug-modulating controllers at specific sites in the body. Indomethacin has been used in the controlled release of drugs because it is a drug that have good interaction with different polymers. The drug is a non-steroidal anti-inflammatory drug used in the treatment of rheumatoid arthritis, osteoarthritis, spondylitis, and other disorders. In this work, PEG 4000 had its chain modified by organic reactions and their derivatives were emulsified to form microparticles using polyvinyl alcohol as an emulsifier. Posteriorly were also incorporated with indomethacin. The samples were characterized to prove the influence of indomethacin on the morphology and thermal behavior of this polymer. The controlled release was performed in the time from 0 to 240 min using the Ultraviolet Spectroscopy to quantify indomethacin released from the polymer matrix for these 4 hours. Releases over the time were satisfactory as concentrations increased over time, which we can conclude that the structural modification of PEG 4000 was beneficial in the release of the indomethacin drug.Item Photoprotective activity and increase of SPF in sunscreen formulation using lyophilized red propolis extracts from Alagoas.(2019) Almeida, Wanessa Aparecida da Silveira; Antunes, Amanda dos Santos; Penido, Ricardo Gonçalves; Correa, Helen Silva da Gloria; Nascimento, Andréa Mendes do; Andrade, Ângela Leão; Santos, Vagner Rodrigues; Cazati, Thiago; Amparo, Tatiane Roquete; Souza, Gustavo Henrique Bianco de; Freitas, Katia Michelle; Santos, Orlando David Henrique dos; Sousa, Lucas Resende Dutra; Santos, Viviane Martins Rebello dosThe excessive sun exposure, coupled with lack of sun protection represents one of the biggest risks to the occurrence of skin cancer and photoaging. Recent strategies for photoprotection have included the incorporation of natural antioxidant and anti-inflammatory compounds, into sunscreens, and the oral administration of natural antioxidant extracts. In this work, we use Brazilian red propolis extract because its antioxidant and anti-inflammatory activities. The aim of this work was to evaluate the sun protection factor and antioxidant activity of different hydroalcoholic extracts of red propolis (70% and 75%) prepared from lyophilized red propolis at room and high temperatures. The sun protection factor in vitro was determined by a spectrophotometric method developed by Mansur. The hydroalcoholic extracts of red propolis incorporated with Filter UVA-UVB 5% Gel Permulem TR-1 presented absorption in the UVB region. Also, the in vitro capacity of the hydroalcoholic extracts of red propolis to increase photoprotective activity of Filter UVA-UVB 5% Gel was evaluated. The hydroalcoholic extracts of red propolis incorporated presented higher values of sun protection factor and showed synergism in the photoprotective activity of Filter UVA-UVB 5% Gel Permulem TR-1. The antioxidant activity and sun protection factor are correlated with total phenolics content of the extracts and the hydroalcoholic extract of red propolis 75% at room temperature was choosen. The formulation developed with Filter UVA-UVB 5% Gel Permulem TR-1 with this hydroalcoholic extract of red propolis showed safe to be applied on the skin according HET-CAM test. Suggests indication of hydroalcoholic extract of red propolis (75% – room temperature) associated to photoprotective formulations for use in photoprotective products.Item Preparation of glass-ionomer cement containing ethanolic Brazilian pepper extract (Schinus terebinthifolius Raddi) fruits : chemical and biological assays.(2020) Pinto, Isabelle Cristina; Seibert, Janaína Brandão; Pinto, Luciano Silva; Santos, Vagner Rodrigues; Sousa, Rafaela F. de; Sousa, Lucas Resende Dutra; Amparo, Tatiane Roquete; Santos, Viviane Martins Rebello dos; Nascimento, Andréa Mendes do; Souza, Gustavo Henrique Bianco de; Vasconcellos, Walison Arthuso; Vieira, Paula Melo de Abreu; Andrade, Ângela LeãoPlants may contain benefcial or potentially dangerous substances to humans. This study aimed to prepare and evaluate a new drug delivery system based on a glass-ionomer-Brazilian pepper extract composite, to check for its activity against pathogenic microorganisms of the oral cavity, along with its in vitro biocompatibility. The ethanolic Brazilian pepper extract (BPE), the glass-ionomer cement (GIC) and the composite GIC-BPE were characterized by scanning electron microscopy, attenuated total refectance Fourier transform infrared spectroscopy (ATR-FTIR), and thermal analysis. The BPE compounds were identifed by UPLC–QTOF–MS/MS. The release profle of favonoids and the mechanical properties of the GIC-BPE composite were assessed. The favonoids were released through a linear mechanism governing the difusion for the frst 48 h, as evidenced by the Mt √ /M∞ relatively to t, at a difusion coefcient of 1.406 × 10–6 cm2 s−1. The ATR-FTIR analysis indicated that a chemical bond between the GIC and BPE components may have occurred, but the compressive strength of GIC-BPE does not difer signifcantly from that of this glass-ionomer. The GIC-BPE sample revealed an ample bacterial activity at non-cytotoxic concentrations for the human fbroblast MRC-5 cells. These results suggest that the prepared composite may represent an alternative agent for endodontic treatment.Item Propostas contextualizadas de práticas de química orgânica para o ensino de biologia.(2021) Alvarenga, Meiry Edivirges; Sousa, Lucas Resende Dutra; Andrade, Ângela Leão; Melo, Tânia Márcia Sacramento; Silva Neto, Leonel Antônio da; Nascimento, Andréa Mendes do; Santos, Viviane Martins Rebello dosEm nossa prática docente, notamos com frequência, o questionamento por parte dos estudantes acerca do motivo pelo qual se estuda Química. É importante ressaltar que a motivação pelo ensino dessa disciplina poderá ser alcançada abandonando-se as aulas baseadas apenas na memorização de nomes e fórmulas e adotando-se abordagens interdisciplinares e contextualizadas. Dessa forma, o presente trabalho visa propor práticas de ensino de química que fortaleçam a motivação dos estudantes de Ciências Biológicas. Com esse objetivo, selecionamos experimentos para serem desenvolvidos em aulas de caráter prático, ofertadas para estudantes do curso de Ciências Biológicas, da Universidade Federal de Ouro Preto. Os experimentos foram testados pelo monitor e os que apresentaram resultados experimentais satisfatórios foram ministrados para tais alunos. Ao final da aula, os alunos responderam um questionário que ajudou a avaliar o grau de satisfação dos mesmos sobre as práticas contextualizadas. Observamos que 50% dos estudantes relataram um maior interesse pela Química Orgânica após as intervenções propostas, pois tiveram uma percepção da relação existente entre preceitos relativos àquele campo do conhecimento e o seu curso de Ciências Biológicas.Item Trypanocidal activity and increased solubility of benznidazole incorporated in PEG 4000 and its derivatives.(2021) Sousa, Lucas Resende Dutra; Azevedo, Maria Luíza Schaefer; Rocha, Dayana F.; Andrade, Ângela Leão; Amparo, Tatiane Roquete; Santos, Orlando David Henrique dos; Seibert, Janaína Brandão; Pereira, Luciano Rodrigues; Vieira, Paula Melo de Abreu; Carneiro, Cláudia Martins; Barboza, Ana Paula Moreira; Neves, Bernardo Ruegger Almeida; Sales Junior, Policarpo Ademar Molina; Murta, Silvane Maria Fonseca; Novack, Kátia Monteiro; Santos, Viviane Martins Rebello dosSelecting a polymer depends on its characteristics, the properties of the drug and of the remaining ingredients in the formulation. The drug, when incorporated into a polymeric matrix, can show several advantages when compared with its conventional form. In this context, this work describes the preparation and characterization of polyethylene glycol (PEG 4000) and its derivative particles loaded with benznidazole, as well as evaluates their trypanocidal activity. In this work, reactions to modify the PEG 4000 polymer and the subsequent incorporation of the benznidazole were made. The nuclear magnetic resonance (NMR) analysis confirmed the efficiency in modifying the PEG chains. The morphology of polymeric films was observed by atomic force microscopy (AFM) and showed considerable changes on the film organization. The acetylation of PEG favored the stability of the system and an increase in the zeta potential from -14.83 to -25.54 mV was observed. Although encapsulation efficiency values between 30.14 and 39.48% were found, the enhanced benznidazole dissolution profile by microparticles enables the use of lower drug concentrations. This fact can be proven by the increased trypanocidal effect of benznidazole when encapsulated in BP3 microparticles. Finally, the high selectivity of the formulations for trypanocidal action guarantees their safety as an alternative for the treatment of the Chagas disease.