Pereira, Michele B. P.Tisi, RenataFietto, Luciano GomesCardoso, Anamaria de SouzaFrança, Mônica M.Carvalho, Fernanda Machado deTrópia, Maria José MagalhãesMartegani, EnzoCastro, Ieso de MirandaBrandão, Rogélio Lopes2012-07-182012-07-182008PEREIRA, M. B. P. et al. Carbonyl cyanidem - chlorophenylhydrazone induced calcium signaling and activation of plasma membrane H1-ATPase in the yeast Saccharomyces cerevisiae. FEMS Yeast Research, v. 8, n.4, p.622-630, jun. 2008. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1111/j.1567-1364.2008.00380.x/pdf>. Acesso em: 18 jul. 2012.15671364http://www.repositorio.ufop.br/handle/123456789/1164The plasma membrane H1-ATPase from Saccharomyces cerevisiae is an enzyme that plays a very important role in the yeast physiology. The addition of protonophores, such as 2,4-dinitrophenol (DNP) and carbonyl cyanide m-chlorophenylhydrazone (CCCP), also triggers a clear in vivo activation of this enzyme. Here, we demonstrate that CCCP-induced activation of the plasma membrane H1-ATPase shares some similarities with the sugar-induced activation of the enzyme. Phospholipase C and protein kinase C activities are essential for this activation process while Gpa2p, a G protein involved in the glucose-induced activation of the ATPase, is not required. CCCP also induces a phospholipase Cdependent increase in intracellular calcium. Moreover, we show that the availability of extracellular calcium is required for CCCP stimulation of H1-ATPase, suggesting a possible connection between calcium signaling and activation of ATPase.en-USDepolarizing compoundsSaccharomyces cerevisiaeArtigo publicado em periodicoO Periódico FEMS Yeast Research concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 500810576.