Caldeira, Tamires GuedesGuimarães, Dênia Antunes SaúdeDezani, André BersaniSerra, Cristina Helena dos ReisSouza, Jacqueline de2019-04-152019-04-152018CALDEIRA, T. G. et al. In silico and in vitro prediction of gastrointestinal absorption from potential drug eremantholide C. Journal of Pharmacy and Pharmacology, v. 69, p. 1468-1476, 2017. Disponível em: <https://onlinelibrary.wiley.com/doi/full/10.1111/jphp.12783>. Acesso em: 25 fev. 2019.20427158http://www.repositorio.ufop.br/handle/123456789/11015Objectives Analysis of the biopharmaceutical properties of eremantholide C, sesquiterpene lactone with proven pharmacological activity and low toxicity, is required to evaluate its potential to become a drug. Methods Preliminary analysis of the physicochemical characteristics of eremantholide C was performed in silico. Equilibrium solubility was evaluated using the shake‐flask method, at 37.0 °C, 100 rpm during 72 h in biorelevant media. The permeability was analysed using parallel artificial membrane permeability assay, at 37.0 °C, 50 rpm for 5 h. The donor compartment was composed of an eremantholide C solution in intestinal fluid simulated without enzymes, while the acceptor compartment consisted of phosphate buffer. Key findings Physicochemical characteristics predicted in silico indicated that eremantholide C has a low solubility and high permeability. In‐vitro data of eremantholide C showed low solubility, with values for the dose/solubility ratio (ml): 9448.82, 10 389.61 e 15 000.00 for buffers acetate (pH 4.5), intestinal fluid simulated without enzymes (pH 6.8) and phosphate (pH 7.4), respectively. Also, it showed high permeability, with effective permeability of 30.4 × 10−6 cm/s, a higher result compared with propranolol hydrochloride (9.23 × 10−6 cm/s). Conclusions The high permeability combined with its solubility, pharmacological activity and low toxicity demonstrate the importance of eremantholide C as a potential drug candidate.en-USrestritoParallel artificial membrane permeability assaySolubilityPermeabilityshake-flaskArtigo publicado em periodicohttps://onlinelibrary.wiley.com/doi/full/10.1111/jphp.12783