Carvalho, Diogo Emerson Leite deOliveira, Katia Mara deBomfim, Larissa MendesSoares, Milena Botelho PereiraBezerra, Daniel PereiraBatista, Alzir AzevedoCorrea, Rodrigo de Souza2020-06-222020-06-222020CARVALHO, D. E. L. et al. Nucleobase derivatives as building blocks to form Ru(II)-based complexes with high cytotoxicity. ACS Omega, v. 5, n. 1, p. 122-130, jan. 2020. Disponível em: <https://pubs.acs.org/doi/10.1021/acsomega.9b01921>. Acesso em: 10 fev. 2020.2470-1343http://www.repositorio.ufop.br/handle/123456789/12376Two new Ru(II)-based complexes containing 2-thiouracil derivatives, known as 2-thiouracil (2TU) and 6-methyl-2-thiouracil (6m2TU), were synthesized using cis,trans-[RuCl2(PPh3)2(bipy)] as a precursor. The obtained compounds with a general formula trans-[Ru(2TU)(PPh3)2(bipy)]PF6 (1) and trans-[Ru(6m2TU)(PPh3)2(bipy)]PF6 (2) were characterized by analytical techniques such as NMR, UV–vis, and IR spectroscopies, elementary analysis, mass spectrometry, and single-crystal X-ray diffraction. Moreover, the investigation of the complexes–DNA interaction were carried out using spectrophotometric titrations and showed that the complexes present a weak interaction with this biomolecule. The compounds were evaluated against HL-60, K-562, HepG2, and B16-F10 cancer cells and against noncancer cells (PBMCs). The results of the biological assay revealed that complex 2 is more promising than complex 1. Finally, the present study suggests that complexes 1 and 2 causes cell death by apoptosis, significantly increasing the percentage of apoptotic HL-60 cells, in which the compounds altered the cell cycle, reducing the cells in G1/G0, G2/M, and S phases.en-USabertoArtigo publicado em periodicoThis is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. Fonte: o PDF do artigo.https://doi.org/10.1021/acsomega.9b01921