Antimitotic and toxicogenetic action of Stevia urticifolia aerial parts on proliferating vegetal and mammalian cells : in vitro and in vivo traditional and replacement methods.

Ferreira, Paulo Michel PinheiroSousa, Ian Jhemes OliveiraMachado, Kamilla NunesSilva Neto, Leonel Antônio daFreitas, Milena Monteiro deSantos, Ingredy Lopes dosRodrigues, Débora Caroline do NascimentoSousa, Rayran Walter Ramos deReis, Antonielly Campinho dosNascimento, Maria Luisa Lima Barreto doMenezes, Ag-Anne Pereira Melo deNascimento, Andréa Mendes doFerreira, José Roberto de OliveiraPeron, Ana PaulaSousa, João Marcelo de Castro e2023-11-172023-11-172022FERREIRA, P. M. P. et al. Antimitotic and toxicogenetic action of Stevia urticifolia aerial parts on proliferating vegetal and mammalian cells: in vitro and in vivo traditional and replacement methods. Journal of Toxicology and Environmental Health-Part A-Current Issues, v. 85, p. 750-766, 2022. Disponível em: <https://www.tandfonline.com/doi/full/10.1080/15287394.2022.2081640>. Acesso em: 01 ago. 2023.1087-2620http://www.repositorio.ufop.br/jspui/handle/123456789/17809Stevia urticifolia Thunb. is an underexploited herb possessing bioactive flavonoids, saponins, and terpenoids. The aim of this study was to examine the antiproliferative and toxicogenetic properties of the ethyl acetate extract from Stevia urticifolia aerial parts (EtAcSur) upon Artemia salina, erythrocytes, Allium cepa and sarcoma 180 cells and fibroblasts, as well as in vivo studies on mice to determine systemic, macroscopic, and behavioral alterations and bone marrow chromosomal damage. The assessment using A. salina larvae and mouse blood cells revealed LC50 and EC50 values of 68.9 and 113.6 µg/ml, respectively. Root growth and mitosis were inhibited by EtAcSur, and chromosomal aberrations were detected only at 100 μg/ml. EtAcSur exhibited potent concentration-dependent viability reduction of S180 and L-929 cells and antioxidant capacity employing ABTS• and DPPH• . No previous in vivo studies were performed before with the EtAcSur. Signals of acute toxicity were not observed at 300 mg/kg. Physiological and toxicological investigations at 25 and 50 mg/mg/day i.p. for 8 days did not markedly change body or organ relative weights, nor patterns of spontaneous locomotor and exploratory activities. In contrast, clastogenic effects on bone marrow were found at 50 mg/mg/day. EtAcSur was found to (1) produce toxicity in microcrustaceans, (2) capacity as free radical scavenger, (3) antimitotic, cytotoxic and clastogenic activties upon vegetal and mammalian cells, and (4) lethality on both tumor and normal murine cells indistinctly. In vivo damage systemic effects were not remarkable and clinical signals of toxicity were not observed, suggesting the significant pharmacological potential of S. urticifolia for the development of antineoplastic agents.en-USrestritoAllium cepaChemopreventionIn vivo toxicityGenotoxicitySarcoma 180Antimitotic and toxicogenetic action of Stevia urticifolia aerial parts on proliferating vegetal and mammalian cells : in vitro and in vivo traditional and replacement methods.Artigo publicado em periodicohttps://www.tandfonline.com/doi/full/10.1080/15287394.2022.2081640https://doi.org/10.1080/15287394.2022.2081640