Gomes, Katia das NevesFreitas, Suzy Magaly Alves Cabral dePais, Thiago MartinsFietto, Juliana Lopes RangelTotola, Antonio HelvecioArantes, Rosa Maria EstevesMartins, AntónioLucas, Cândida Manuel Ribeiro SimõesSchuller, DoritCasal, MargaridaCastro, Ieso de MirandaFietto, Luciano GomesRogelio, Lopes Brandão2017-03-142017-03-142005GOMES, K. das N. et al. Deficiency of Pkc1 activity affects glycerol metabolism in Saccharomyces cerevisiae. Fems Yeast Research Amsterdan, v. 5, n. 8, p.767-776, 2005. Disponível em: <http://femsyr.oxfordjournals.org/content/5/8/767.long>. Acesso em: 10 jan. 2017.1567-1364http://www.repositorio.ufop.br/handle/123456789/7375Protein kinase C is apparently involved in the control of many cellular systems: the cell wall integrity pathway, the synthesis of ribosomes, the appropriated reallocation of transcription factors under specific stress conditions and also the regulation of N-glycosylation activity. All these observations suggest the existence of additional targets not yet identified. In the context of the control of carbon metabolism, previous data had demonstrated that Pkc1p might play a central role in the control of cellular growth and metabolism in yeast. In particular, it has been suggested that it might be involved in the derepression of genes under glucose-repression by driving an appropriated subcellular localization of transcriptional factors, such as Mig1p. In this work, we show that a pkc1D mutant is unable to grow on glycerol because it cannot perform the derepression of the GUT1 gene that encodes glycerol kinase. Additionally, active transport is also partially affected. Using this phenotype, we were able to isolate a new pkc1D revertant. We also isolated two transformants identified as the nuclear exportin Msn5 and the histone deacetylase Hos2 extragenic suppressors of this mutation. Based on these results, we postulate that Pkc1p may be involved in the control of the cellular localization and/or regulation of the activity of nuclear proteins implicated in gene expression.en-USrestritoProtein kinase CGlucose repressionGlycerol transportGlycerol metabolismArtigo publicado em periodicohttp://femsyr.oxfordjournals.org/content/5/8/767.longhttps://doi.org/10.1016/j.femsyr.2005.01.003