Sanchez, Angelica BianchiniGarcia, Camila Carrião MachadoFreitas, Florêncio PortoBatista, Guilherme LopesLopes, Fernando SilvaCarvalho, Victor HenriqueRonsein, Graziella ElizaGutz, Ivano Gebhardt RolfMascio, Paolo DiMedeiros, Marisa Helena Gennari de2019-04-042019-04-042018SANCHEZ, A. B. et al. DNA ddduct formation in the lungs and brain of rats exposed to low concentrations of [13C2]‑Acetaldehyde. Chemical Research in Toxicology Article, v. 31, n. 5, p. 332–339, 2018. Disponível em: <https://pubs.acs.org/doi/10.1021/acs.chemrestox.8b00016>. Acesso em: 22 fev. 2019.15205010http://www.repositorio.ufop.br/handle/123456789/10946Air pollution is a major environmental risk for human health. Acetaldehyde is present in tobacco smoke and vehicle exhaust. In this study, we show that [13C2]-acetaldehyde induces DNA modification with the formation of isotopically labeled 1,N2-propano-2′-deoxyguanosine adducts in the brain and lungs of rats exposed to concentrations of acetaldehyde found in the atmosphere of megacities. The adduct, with the addition of two molecules of isotopically labeled acetaldehyde [13C4]-1,N2-propano-dGuo, was detected in the lung and brain tissues of exposed rats by micro-HPLC/MS/MS. Structural confirmation of the products was unequivocally performed by nano-LC/ESI+-HRMS3 analyses. DNA modifications induced by acetaldehyde have been regarded as a key factor in the mechanism of mutagenesis and may be involved in the cancer risks associated with air pollution.en-USrestritoArtigo publicado em periodicohttps://pubs.acs.org/doi/10.1021/acs.chemrestox.8b00016