Santana, Marcos Aurélio deSantos, Alexandre Martins CostaOliveira, Marcelo EurípedesOliveira, Jamil Silvano deBabá, Élio HideoSantoro, Marcelo MatosAndrade, Milton Hércules Guerra de2015-03-102015-03-102008SANTANA, M. A. de et al. A novel and efficient and low-cost methodology for purification of Macrotyloma axillare (Leguminosae) seed lectin. International Journal of Biological Macromolecules, v. 43, p. 352-358, 2008. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0141813008001578>. Acesso em: 08 nov. 2014.0141-8130http://www.repositorio.ufop.br/handle/123456789/4576The N-acetyl-galactosamine specific lectin fromMacrotyloma axillare seeds (LMA)was purified by precipitation and ion exchange chromatography. The LMA 0.2 mol L−1 fraction showed hemagglutinating activity on erythrocytes A1. The results for molecular mass determinations were about 28 kDa. The LMA pHdependent assays showed best hemagglutinating activity at pH 6.0–8.0; being decreased at acidic/alkaline conditions and by EDTA treatment. LMA is a tetramer at pH 8.2 and a dimer at pH 4.0. Human erythrocytes from ABO system confirmed the A1 specificity for LMA. This new methodology is useful and easy, with low costs, for lectin purification in large amounts.en-USMacrotyloma axillareLectinPurificationAntigenArtigo publicado em periodicoO periódico International Journal of Biological Macromolecules concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3546550273168.https://doi.org/10.1016/j.ijbiomac.2008.07.011