Gomes, Matheus de SouzaCabral, Fernanda JankuPassos, Liana Konovaloff JannottiCarvalho, OmarRodrigues, VanderleiBabá, Élio HideoCota, Renata Guerra de Sá2015-03-062015-03-062009GOMES, M. de S. et al. Preliminary analysis of miRNA pathway in Schistosoma mansoni. Parasitology International, v. 58, p. 61-68, 2009. Disponível em: <http://www.sciencedirect.com/science/article/pii/S1383576908001165>. Acesso em: 08 nov. 2014.1383-5769http://www.repositorio.ufop.br/handle/123456789/4552RNA silencing refers to a series of nuclear and cytoplasmatic processes involved in the post-transcriptional regulation of gene expression or post-transcriptional gene silencing (PTGS), either by sequence-specific mRNA degradation or by translational arrest. The best characterized small RNAs are microRNAs (miRNAs), which predominantly perform gene silencing through post-transcriptional mechanisms. In this work we used bioinformatic approaches to identify the parasitic trematode Schistosoma mansoni sequences that are similar to enzymes involved in the post-transcriptional gene silencing mediated by miRNA pathway.We used amino acid sequences of well-known proteins involved in the miRNA pathway against S. mansoni genome and transcriptome databases identifying a total of 13 putative proteins in the parasite. In addition, the transcript levels of SmDicer1 and SmAgo2/3/4 were identified by qRT-PCR using cercariae, adult worms, eggs and in vitro cultivated schistosomula. Our results showed that the SmDicer1 and SmAgo2/3/4 are differentially expressed during schistosomula development, suggesting that the miRNA pathway is regulated at the transcript level and therefore may control gene expression during the life cycle of S. mansoni.en-USSchistosoma mansoniGene expressionPost-transcriptional gene silencingArtigo publicado em periodicoO periódico Parasitology International concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3547120424363.https://doi.org/10.1016/j.parint.2008.10.002