Pereira, Isabela Amorim GonçalvesMendonça, Débora Vasconcelos CostaTavares, Grasiele de Sousa VieiraLage, Daniela PagliaraRamos, Fernanda FonsecaSilva, João Augusto Oliveira daAntinarelli, Luciana Maria RibeiroMachado, Amanda SanchezCarvalho, Lívia MendesCarvalho, Ana Maria Ravena SeverinoSalustiano, Iorrana VieiraReis, Thiago Alves Rosa dosBandeira, Raquel SoaresSilva, Alessandra M.Martins, Vivian TamiettiChávez Fumagalli, Miguel AngelHumbert, Maria VictoriaRoatt, Bruno MendesDuarte, Mariana CostaSouza, Daniel MenezesCoimbra, Elaine SoaresLeite, João Paulo VianaCoelho, Eduardo Antônio FerrazGonçalves, Denise Utsch2021-09-302021-09-302020PEREIRA, I. A. G. et al. Parasitological and immunological evaluation of a novel chemotherapeutic agent against visceral leishmaniasis. Parasite Immunology, v. 42, n. 12, artigo e12784, dez. 2020. Disponível em: <https://onlinelibrary.wiley.com/doi/abs/10.1111/pim.12784>. Acesso em: 10 jun. 2021.1365-3024http://www.repositorio.ufop.br/jspui/handle/123456789/13830Aims: Treatment for visceral leishmaniasis (VL) is hampered by the toxicity and/or high cost of drugs, as well as by emergence of parasite resistance. Therefore, there is an urgent need for new antileishmanial agents. Methods and Results: In this study, the antileishmanial activity of a diprenylated flavonoid called 5,7,3,4’-tetrahydroxy-6,8-diprenylisoflavone (CMt) was tested against Leishmania infantum and L amazonensis species. Results showed that CMt presented selectivity index (SI) of 70.0 and 165.0 against L infantum and L amazonensis promastigotes, respectively, and of 181.9 and 397.8 against respective axenic amastigotes. Amphotericin B (AmpB) showed lower SI values of 9.1 and 11.1 against L infantum and L amazonensis promastigotes, respectively, and of 12.5 and 14.3 against amastigotes, respectively. CMt was effective in the treatment of infected macrophages and caused alterations in the parasite mitochondria. L infantum-infected mice treated with miltefosine, CMt alone or incorporated in polymeric micelles (CMt/Mic) presented significant reductions in the parasite load in distinct organs, when compared to the control groups. An antileishmanial Th1-type cellular and humoral immune response were developed one and 15 days after treatment, with CMt/Mic-treated mice presenting a better protective response. Conclusion: Our data suggest that CMt/Mic could be evaluated as a chemotherapeutic agent against VL.en-USrestritoDelivery systemsFlavonoidsImmune responseMiltefosineTreatmentArtigo publicado em periodicohttps://onlinelibrary.wiley.com/doi/abs/10.1111/pim.12784https://doi.org/10.1111/pim.12784