Castro, Ieso de MirandaDias, Maria da Conceição Loureiro2017-03-202017-03-201994CASTRO, I. de M.; DIAS, M. C. L. Utilization of lactic acid by Fusarium oxysporum var. lini: regulation of transport and metabolism. Applied And Environmental Microbiology, v. 60, p.102-105, 1994. Disponível em: <http://aem.asm.org/content/60/1/102.long>. Acesso em: 10 jan. 2017.1098-5336http://www.repositorio.ufop.br/handle/123456789/7395Lactic acid was transported in Fusarium oxysporum var. lini ATCC 10960 by a saturable transport system that had a half-saturation constant of 56.6 7.5 FLM and a maximum velocity of 0.61 0.10 mmol h-1 g-1 (dry weight) at 26°C and pH 5.0. This transport system was inducible and was not expressed in the presence of a repressing substrate. Evidence is presented that the anionic form lactate- was taken up by the cells. Propionic, acetic, pyruvic, and bromoacetic acids but not succinic acid competitively inhibited the transport of lactic acid. Bromoacetic acid, which was not metabolized, was taken up to a steady-state level when intracellular and extracellular concentrations were identical, indicating that the transport system was not accumulative. The enzymatic activity that was physiologically more relevant in the metabolism of lactic acid was lactate: ferricytochrome c oxidase. This enzyme did not exhibit stereospecifity and was induced by lactic acid.en-USrestritoArtigo publicado em periodicohttp://aem.asm.org/content/60/1/102.long