Sauvain, Jean-JacquesEdmé, Jean LouisWild, PascalSuárez, Gina WirzBezerra, Olívia Maria de Paula AlvesSilva, André Talvani Pedrosa daAlgranti, EduardoCarneiro, Ana Paula ScaliaChérot-Kornobis, NathalieSobaszek, AnnieHulo, Sébastien2021-09-232021-09-232020SAUVAIN, J. J. et al. Does exposure to inflammatory particles modify the pattern of anion in exhaled breath condensate?. Journal of Breath Research, v. 14, id 026005, 2020. Disponível em: <https://iopscience.iop.org/article/10.1088/1752-7163/ab5d88>. Acesso em: 10 jun. 2021.1752-7163http://www.repositorio.ufop.br/jspui/handle/123456789/13795Exposure to environmental and occupational particulate matter(PM)induces health effects on the cardio-pulmonary system. In addition, associations between exposure to PM and metabolic syndromes like diabetes mellitus or obesity are now emerging in the literature. Collection of exhaled breath condensate (EBC) is an appealing non-invasive technique to sample pulmonary fluids. This hypothesis-generating study aims to (1) validate an ion chromatography method allowing the robust determination of different metabolism-related molecules(lactate, formate, acetate, propionate, butyrate, pyruvate, nitrite, nitrate)in EBC; (2) apply this method to EBC samples collected from workers exposed to quartz (a known inflammatory particle), to soapstone (a less inflammatory particle than quartz), as well as to controls. A multi-compound standard solution was used to determine the linearity range, detection limit, repeatability and bias from spiked EBC. The biological samples were injected without further treatment into an ion chromatograph with a conductivity detector. RTube® were used for field collection of EBC from 11 controls, 55 workers exposed to soapstone and 12 volunteers exposed to quartz dust. The analytical method used proved to be adequate for quantifying eight anions in EBC samples. Its sub-micromolar detection limits and repeatability, combined with a very simple sample preparation, allowed an easy and fast quantification of different glycolysis or nitrosative stress metabolites. Using multivariate discriminant analysis to maximize differences between groups, we observed a different pattern of anions with a higher formate/acetate ratio in the EBC samples for quartz exposed workers compared to the two other groups. We hypothesize that a modification of the metabolic signature could be induced by exposure to inflammatory particles like quartz and might be observed in the EBC via a change in the formate/acetate ratio.en-USabertoIon chromatographyMetabolismSoapstoneQuartzSmall chain fatty acidsArtigo publicado em periodicoOriginal content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. Fonte: o PDF do artigo.https://doi.org/10.1088/1752-7163/ab5d88