Abreu, Fabiano Carlos Pinto dePereira, Roberta VercianoOliveira, Victor Fernandes deGomes, Matheus de SouzaPassos, Liana Konovaloff JannottiBorges, William de CastroCota, Renata Guerra de Sá2017-06-222017-06-222013ABREU, F. C. P. de et al. Characterization of export receptor exportins (XPOs) in the parasite Schistosoma mansoni. Parasitology Research, v. 1, p. 1-9, 2013. Disponível em: <https://link.springer.com/article/10.1007%2Fs00436-013-3606-x>. Acesso em: 23 fev. 2017.1432-1955http://www.repositorio.ufop.br/handle/123456789/8050Several proteins and different species of RNA that are produced in the nucleus are exported through the nuclear pore complexes, which require a family of conserved nuclear export receptors called exportins (XPOs). It has been reported that the XPOs (XPO1, XPO5, and XPOT) are directly involved in the transport processes of noncoding RNAs from the nucleus to the cytoplasm and/or from cytoplasm to the nucleus. All three genes are present in fungi, plants, and deuterostome metazoans. However, protostome metazoan species lack one of the three genes across evolution. In this report, we have demonstrated that all three XPO proteins are present in the parasite protostome Schistosoma mansoni . As this parasite has a complex life cycle presenting several stages in different hosts and environments, implying a differential gene regulation, we proposed a genomic analysis of XPOs to validate their annotation. The results showed the conservation of exportin family members and gene duplication events in S. mansoni .We performed quantitative RT-PCR, which revealed an upregulation of SmXPO1 in 24 h schistosomula (sixfold when compared with cercariae), and similar transcription levels were observed for SmXPO5 and SmXPOT in all the analyzed stages. These three XPO proteins have been identified for the first time in the protostome clade, which suggests a higher complexity in RNA transport in the parasite S. mansoni. Taken together, these results suggest that RNA transport by exportins might control cellular processes during cercariae, schistosomula, and adult worm development.en-USrestritoArtigo publicado em periodicohttps://link.springer.com/article/10.1007%2Fs00436-013-3606-xhttps://doi.org/10.1007/s00436-013-3606-x