Gomes, Sttefany VianaDias, Bruna VidalMachado Júnior, Pedro AlvesPereira, Renata RebecaSouza, Débora Maria Soares deBreguez, Gustavo SilveiraLima, Wanderson Geraldo deMagalhães, Cíntia Lopes de BritoCangussú, Silvia DantasSilva, André Talvani Pedrosa daQueiroz, Karina Barbosa deCalsavara, Allan Jefferson CruzCosta, Daniela Caldeira2023-10-112023-10-112023GOMES, S. V. et al. High-fat diet increases mortality and intensifies immunometabolic changes in septic mice. Journal of Nutritional Biochemistry, v. 116, artigo 109315, jun. 2023. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0955286323000499>. Acesso em: 01 ago. 2023.0955-2863http://www.repositorio.ufop.br/jspui/handle/123456789/17559Immunometabolic changes in the liver and white adipose tissue caused by high-fat (HF) diet intake may worse metabolic adaptation and protection against pathogens in sepsis. We investigate the effect of chronic HF diet (15 weeks) on mortality and immunometabolic responses in female mice after sepsis induced by cecum ligation and perforation (CLP). At week 14, animals were divided into four groups: sham C diet, sepsis C diet (C-Sp), sham HF diet (HF-Sh) and sepsis HF diet (HF-Sp). The surviving animals were euthanized on the 7th day. The HF diet decreased survival rate (58.3% vs. 76.2% C-Sp group), increased serum cytokine storm (IL-6 [1.41 ×; vs. HF-Sh], IL-1β [1.37 ×; vs. C-Sp], TNF [1.34 ×; vs. C-Sp and 1.72 ×; vs. HF-Sh], IL-17 [1.44 ×; vs. HF-Sh], IL-10 [1.55 ×; vs. C-Sp and 1.41 ×; HF-Sh]), white adipose tissue inflammation (IL-6 [8.7 ×; vs. C-Sp and 2.4 ×; vs. HF-Sh], TNF [5 ×; vs. C-Sp and 1.7 ×; vs. HF-Sh], IL-17 [1.7 ×; vs. C-Sp], IL-10 [7.4 ×; vs. C-Sp and 1.3 ×; vs. HF-Sh]), and modulated lipid metabolism in septic mice. In the HF-Sp group liver's, we observed hepatomegaly, hydropic degeneration, necrosis, an increase in oxidative stress (reduction of CAT activity [−81.7%; vs. HF-Sh]; increase MDA levels [82.8%; vs. HF-Sh], and hepatic IL-6 [1.9 ×; vs. HF-Sh], and TNF [1.3 × %; vs. HF-Sh]) production. Furthermore, we found a decrease in the total number of inflammatory, mononuclear cells, and in the regenerative processes, and binucleated hepatocytes in a HF-Sp group livers. Our results suggested that the organism under metabolic stress of a HF diet during sepsis may worsen the inflammatory landscape and hepatocellular injury and may harm the liver regenerative process.en-USrestritoHigh-fat dietSepsisInfectionImmunometabolismOxidative stressArtigo publicado em periodicohttps://www.sciencedirect.com/science/article/pii/S0955286323000499https://doi.org/10.1016/j.jnutbio.2023.109315