Vale, Isabela Natália Pascoal Campos doSaliba, Juliana WilkeFonseca, Giuliana Schmidt FrançaPascoal, Vanessa Peruhype MagalhãesAraújo, Fernanda Fortes deXavier, Marcelo Antônio PascoalCarvalho, Andréa Teixeira deCampos, Fernanda Magalhães FreireAndrade, Mariléia ChavesLula, Jamille FernandesReis, Alexandre BarbosaLemos, Elenice MoreiraCarvalho, Sílvio Fernando Guimarães deOliveira, EdwardMartins Filho, Olindo Assis2021-09-032021-09-032020VALE, I. N. P. C. do et al. Laboratorial algorithm for serological diagnosis of visceral leishmaniasis using rK39-ICT, DAT-LPC and FC-Simplex IgG1. Journal of Immunological Methods, v. 480, p. 112765, 2020. Disponível em: <https://www.sciencedirect.com/science/article/abs/pii/S002217592030034X?via%3Dihub>. Acesso em: 10 jun. 2021.0022-1759http://www.repositorio.ufop.br/jspui/handle/123456789/13646The performance of distinct serological tests (rK39-ICT, IFAT, DAT-LPC, FC-Simplex IgG1) was assessed and a laboratorial algorithm was proposed for accurate diagnosis of VL. DAT-LPC and FC-Simplex IgG1 showed outstanding accuracy (AUC = 0.93) to identify VL patients. The use of a sequential serological algorithm (rK39-ICT screening followed by DAT-LPC or FC-Simplex IgG1) improved the global accuracy for VL (97.2%) diagnosis. An alternative approach for diagnosis of VL has been also assessed for interchangeable use of serum/whole blood lysate samples in DAT-LPC and FC-Simplex IgG1. Our data showed an outstanding agreement for the results obtained with whole blood lysate samples as compared to serum samples (DAT-LPC =100%; FC-Simplex IgG1 = 99%). Together, these findings provide insights to improve the current overall accuracy of VL diagnosis and present innovative laboratorial tests and alternative samples from use in public health services.en-USrestritoArtigo publicado em periodicohttps://www.sciencedirect.com/science/article/abs/pii/S002217592030034X?via%3Dihubhttps://doi.org/10.1016/j.jim.2020.112765