Acyl homoserine lactone‑based quorum sensing stimulates biofilm formation by Salmonella Enteritidis in anaerobic conditions.

dc.contributor.authorAlmeida, Felipe Alves de
dc.contributor.authorPimentel Filho, Natan de Jesus
dc.contributor.authorPinto, Uelinton Manoel
dc.contributor.authorMantovani, Hilário Cuquetto
dc.contributor.authorOliveira, Leandro Licursi de
dc.contributor.authorVanetti, Maria Cristina Dantas
dc.date.accessioned2017-10-24T14:30:24Z
dc.date.available2017-10-24T14:30:24Z
dc.date.issued2017
dc.description.abstractQuorum sensing regulates a variety of phenotypes in bacteria including the production of virulence factors. Salmonella spp. have quorum sensing systems mediated by three autoinducers (AI-1, AI-2, and AI-3). The AI-1-mediated system is incomplete in that the bacterium relies on the synthesis of signaling molecules by other microorganisms. This study aimed to evaluate the influence of the AI-1 N-dodecanoyl-DL-homoserine lactone (C12- HSL) on the growth, motility, adhesion, and biofilm formation of Salmonella enterica serovar Enteritidis PT4 578 on a polystyrene surface. Experiments were conducted at 37 °C in anaerobic tryptone soy broth supplemented with C12-HSL and/or a mixture of four synthetic furanones, at the concentration of 50 nM each. The planktonic growth, adhesion, swarming, and twitching motility were not altered in the presence of C12-HSL and/or furanones under anaerobic conditions. However, C12-HSL induced biofilm formation after 36 h of cultivation as determined by quantification of biofilm formation, by enumeration of adhered cells to polystyrene coupons, and finally by imaging the presence of multilayered cells on an epifluorescence microscope. When furanones were present in the medium, an antagonistic effect against C12-HSL on the biofilm development was observed. The results demonstrate an induction of biofilm formation in Salmonella Enteritidis by AI-1 under anaerobic conditions. Considering that Salmonella does not produce AI-1 but respond to it, C12-HSL synthesized by other bacterial species could trigger biofilm formation by this pathogen in conditions that are relevant for its pathogenesis.pt_BR
dc.identifier.citationALMEIDA, F. A. de et al. Acyl homoserine lactone‑based quorum sensing stimulates biofilm formation by Salmonella Enteritidis in anaerobic conditions. Archives of Microbiology, v. 199, p. 475-486, 2017. Disponível em: <https://link.springer.com/article/10.1007%2Fs00203-016-1313-6>. Acesso em: 29 ago. 2017.pt_BR
dc.identifier.doihttps://doi.org/10.1007/s00203-016-1313-6
dc.identifier.issn1432-072X
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/9028
dc.identifier.uri2https://link.springer.com/article/10.1007%2Fs00203-016-1313-6pt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectAdhesionpt_BR
dc.subjectAutoinducerpt_BR
dc.subjectBiofilmpt_BR
dc.subjectCellular communicationpt_BR
dc.subjectFuranonept_BR
dc.titleAcyl homoserine lactone‑based quorum sensing stimulates biofilm formation by Salmonella Enteritidis in anaerobic conditions.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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