Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection.
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2017
Autores
Alessio, Glaucia Diniz
Araújo, Fernanda Fortes de
Côrtes, Denise Fonseca
Sales Júnior, Policarpo Ademar
Lima, Daniela Cristina
Gomes, Matheus de Souza
Amaral, Laurence Rodrigues do
Xavier, Marcelo Antônio Pascoal
Carvalho, Andréa Teixeira de
Martins Filho, Olindo Assis
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Distinct Trypanosoma cruzi genotypes have been considered relevant for patient management
and therapeutic response of Chagas disease. However, typing strategies for genotype-
specific serodiagnosis of Chagas disease are still unavailable and requires
standardization for practical application. In this study, an innovative TcI/TcVI/TcII Chagas
Flow ATE-IgG2a technique was developed with applicability for universal and genotypespecific
diagnosis of T. cruzi infection. For this purpose, the reactivity of serum samples
(percentage of positive fluorescent parasites-PPFP) obtained from mice chronically infected
with TcI/Colombiana, TcVI/CL or TcII/Y strain as well as non-infected controls were determined
using amastigote-AMA, trypomastigote-TRYPO and epimastigote-EPI in parallel
batches of TcI, TcVI and TcII target antigens. Data demonstrated that ªα-TcII-TRYPO/
1:500, cut-off/PPFP = 20%º presented an excellent performance for universal diagnosis of
T. cruzi infection (AUC = 1.0, Se and Sp = 100%). The combined set of attributes ªα-TcITRYPO/
1:4,000, cut-off/PPFP = 50%º, ªα-TcII-AMA/1:1,000, cut-off/PPFP = 40%º and ªα-
TcVI-EPI/1:1,000, cut-off/PPFP = 45%º showed good performance to segregate infections
with TcI/Colombiana, TcVI/CL or TcII/Y strain. Overall, hosts infected with TcI/Colombiana
and TcII/Y strains displayed opposite patterns of reactivity with ªα-TcI TRYPOº and ªα-TcII
AMAº. Hosts infected with TcVI/CL strain showed a typical interweaved distribution pattern.
The method presented a good performance for genotype-specific diagnosis, with global
accuracy of 69% when the population/prototype scenario include TcI, TcVI and TcII infections
and 94% when comprise only TcI and TcII infections. This study also proposes a
receiver operating reactivity panel, providing a feasible tool to classify serum samples from hosts infected with distinct T. cruzi genotypes, supporting the potential of this method for
universal and genotype-specific diagnosis of T. cruzi infection.
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ALESSIO, G. D. et al. Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection. PLoS Neglected Tropical Diseases, v. 11, p. e0005444-24, 2017. Disponível em: <http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0005444>. Acesso em: 29 ago. 2017.