Pristimerin isolated from Salacia crassifolia (Mart. Ex. Schult.) G. Don. (Celastraceae) roots as a potential antibacterial agent against Staphylococcus aureus.

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dc.contributor.authorNizer, Waleska Stephanie da Cruz
dc.contributor.authorFerraz, Ariane Coelho
dc.contributor.authorMoraes, Thaís de Fátima Silva
dc.contributor.authorLima, William Gustavo de
dc.contributor.authorSantos, Josana Pereira dos
dc.contributor.authorDuarte, Lucienir Pains
dc.contributor.authorFerreira, Jaqueline Maria Siqueira
dc.contributor.authorMagalhães, Cíntia Lopes de Brito
dc.contributor.authorVieira Filho, Sidney Augusto
dc.contributor.authorAndrade, Ana Cláudia dos Santos Pereira
dc.contributor.authorRodrigues, Rodrigo Araújo Lima
dc.contributor.authorAbrahão, Jônatas Santos
dc.contributor.authorMagalhães, José Carlos de
dc.date.accessioned2021-10-18T17:28:17Z
dc.date.available2021-10-18T17:28:17Z
dc.date.issued2021pt_BR
dc.description.abstractEthnopharmacological relevance: Pristimerin is a triterpenoid considered the main component of Salacia crassifolia extracts. This terpene has shown promising antitumor, anti-inflammatory, and antimicrobial effects. Likewise, S. crassifolia has been used in traditional medicine to treat cancer and as an antimicrobial and anti-inflammatory agent. Aim of the study: This study aimed to evaluate the antibacterial activity of the hexane extract of Salacia crassifolia roots (HER) and its isolate, pristimerin, against pathogenic bacteria. Materials and methods: First, we evaluated the spectrum of action of HER and pristimerin by the determination of the minimum inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC). Subsequently, we analyzed the time-kill curve of these plant-derived compounds against Staphylococcus aureus. Then, we examined their mode of action by three different assays: the crystal violet methodology, the release of intracellular material, and transmission electron microscopy methods (TEM). Finally, we evaluated the effect of HER and pristimerin on the pre-formed biofilm of S. aureus by the crystal violet assay, the synergistic effect by the checkerboard method, the cytotoxicity against Vero cells, and the in silico activity using the online software PASS. Results: HER and pristimerin presented a narrow spectrum of action against Gram-positive bacteria (MIC 0.195–25 μg/mL), and their primary mode of action is the alteration of membrane permeability of S. aureus. Our results show that the compounds disrupted the pre-formed biofilm of S. aureus in a dose-dependent manner. Furthermore, HER and pristimerin presented a significant synergic effect after the combination with well-known antibiotics, which was associated with the ability of these phytomedicines to change membrane permeability. Regarding the cytotoxic effect, the selective index (SI) of HER ranged from 0.37 to 11.86, and the SI of pristimerin varied from 0.24 to 30.87, according to the bacteria tested. Conclusions: Overall, HER and pristimerin showed a promising antibacterial effect in vitro through the alteration of membrane permeability of S. aureus.pt_BR
dc.identifier.citationNIZER, W. S. da C. et al. Pristimerin isolated from Salacia crassifolia (Mart. Ex. Schult.) G. Don. (Celastraceae) roots as a potential antibacterial agent against Staphylococcus aureus. Journal of Ethnopharmacology, v. 266, artigo 113423, 2021. Disponível em: <https://www.sciencedirect.com/science/article/abs/pii/S0378874120333080>. Acesso em: 10 jun. 2021.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.jep.2020.113423pt_BR
dc.identifier.issn0378-8741
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/13878
dc.identifier.uri2https://www.sciencedirect.com/science/article/abs/pii/S0378874120333080pt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectSynergic effectpt_BR
dc.titlePristimerin isolated from Salacia crassifolia (Mart. Ex. Schult.) G. Don. (Celastraceae) roots as a potential antibacterial agent against Staphylococcus aureus.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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