Deletion of pila, a minor pilin-like Gene, from Xanthomonas citri subsp. Citri influences bacterial physiology and pathogenesis.
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2016
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Type IV pili (Tfp) are widely distributed adhesins
of bacterial surfaces. In plant pathogenic bacteria, Tfp
are involved in host colonization and pathogenesis. Xanthomonas
citri subsp. citri (Xcc) is the phytopathogen
responsible for citrus canker disease. In this work, three
Tfp structural genes, fimA, fimA1, and pilA from Xcc were
studied. A pilA mutant strain from Xcc (XccDpilA) was
constructed and differences in physiological features, such
as motilities, adhesion, and biofilm formation, were
observed. A structural study of the purified Tfp fractions
from Xcc wild-type and XccDpilA showed that pilins are
glycosylated in both strains and that FimA and FimA1 are
the main structural components of the pili. Furthermore,
smaller lesion symptoms and reduced bacterial growth
were produced by XccDpilA in orange plants compared to
the wild-type strain. These results indicate that the minor
pilin-like gene, pilA, is involved in Tfp performance during
the infection process.
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PETROCELLI, S. et al. Deletion of pila, a minor pilin-like Gene, from Xanthomonas citri subsp. Citri influences bacterial physiology and pathogenesis. Current Microbiology, v. 73, p. 904-914, 2016. Disponível em: <https://link.springer.com/article/10.1007%2Fs00284-016-1138-1>. Acesso em: 15 set. 2017.