A novel and efficient and low-cost methodology for purification of Macrotyloma axillare (Leguminosae) seed lectin.

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dc.contributor.authorSantana, Marcos Aurélio de
dc.contributor.authorSantos, Alexandre Martins Costa
dc.contributor.authorOliveira, Marcelo Eurípedes
dc.contributor.authorOliveira, Jamil Silvano de
dc.contributor.authorBabá, Élio Hideo
dc.contributor.authorSantoro, Marcelo Matos
dc.contributor.authorAndrade, Milton Hércules Guerra de
dc.date.accessioned2015-03-10T13:56:43Z
dc.date.available2015-03-10T13:56:43Z
dc.date.issued2008
dc.description.abstractThe N-acetyl-galactosamine specific lectin fromMacrotyloma axillare seeds (LMA)was purified by precipitation and ion exchange chromatography. The LMA 0.2 mol L−1 fraction showed hemagglutinating activity on erythrocytes A1. The results for molecular mass determinations were about 28 kDa. The LMA pHdependent assays showed best hemagglutinating activity at pH 6.0–8.0; being decreased at acidic/alkaline conditions and by EDTA treatment. LMA is a tetramer at pH 8.2 and a dimer at pH 4.0. Human erythrocytes from ABO system confirmed the A1 specificity for LMA. This new methodology is useful and easy, with low costs, for lectin purification in large amounts.pt_BR
dc.identifier.citationSANTANA, M. A. de et al. A novel and efficient and low-cost methodology for purification of Macrotyloma axillare (Leguminosae) seed lectin. International Journal of Biological Macromolecules, v. 43, p. 352-358, 2008. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0141813008001578>. Acesso em: 08 nov. 2014.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.ijbiomac.2008.07.011
dc.identifier.issn0141-8130
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/4576
dc.language.isoen_USpt_BR
dc.rights.licenseO periódico International Journal of Biological Macromolecules concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3546550273168.pt_BR
dc.subjectMacrotyloma axillarept_BR
dc.subjectLectinpt_BR
dc.subjectPurificationpt_BR
dc.subjectAntigenpt_BR
dc.titleA novel and efficient and low-cost methodology for purification of Macrotyloma axillare (Leguminosae) seed lectin.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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