Preliminary analysis of miRNA pathway in Schistosoma mansoni.
dc.contributor.author | Gomes, Matheus de Souza | |
dc.contributor.author | Cabral, Fernanda Janku | |
dc.contributor.author | Passos, Liana Konovaloff Jannotti | |
dc.contributor.author | Carvalho, Omar | |
dc.contributor.author | Rodrigues, Vanderlei | |
dc.contributor.author | Babá, Élio Hideo | |
dc.contributor.author | Cota, Renata Guerra de Sá | |
dc.date.accessioned | 2015-03-06T18:36:01Z | |
dc.date.available | 2015-03-06T18:36:01Z | |
dc.date.issued | 2009 | |
dc.description.abstract | RNA silencing refers to a series of nuclear and cytoplasmatic processes involved in the post-transcriptional regulation of gene expression or post-transcriptional gene silencing (PTGS), either by sequence-specific mRNA degradation or by translational arrest. The best characterized small RNAs are microRNAs (miRNAs), which predominantly perform gene silencing through post-transcriptional mechanisms. In this work we used bioinformatic approaches to identify the parasitic trematode Schistosoma mansoni sequences that are similar to enzymes involved in the post-transcriptional gene silencing mediated by miRNA pathway.We used amino acid sequences of well-known proteins involved in the miRNA pathway against S. mansoni genome and transcriptome databases identifying a total of 13 putative proteins in the parasite. In addition, the transcript levels of SmDicer1 and SmAgo2/3/4 were identified by qRT-PCR using cercariae, adult worms, eggs and in vitro cultivated schistosomula. Our results showed that the SmDicer1 and SmAgo2/3/4 are differentially expressed during schistosomula development, suggesting that the miRNA pathway is regulated at the transcript level and therefore may control gene expression during the life cycle of S. mansoni. | pt_BR |
dc.identifier.citation | GOMES, M. de S. et al. Preliminary analysis of miRNA pathway in Schistosoma mansoni. Parasitology International, v. 58, p. 61-68, 2009. Disponível em: <http://www.sciencedirect.com/science/article/pii/S1383576908001165>. Acesso em: 08 nov. 2014. | pt_BR |
dc.identifier.doi | https://doi.org/10.1016/j.parint.2008.10.002 | |
dc.identifier.issn | 1383-5769 | |
dc.identifier.uri | http://www.repositorio.ufop.br/handle/123456789/4552 | |
dc.language.iso | en_US | pt_BR |
dc.rights.license | O periódico Parasitology International concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3547120424363. | pt_BR |
dc.subject | Schistosoma mansoni | pt_BR |
dc.subject | Gene expression | pt_BR |
dc.subject | Post-transcriptional gene silencing | pt_BR |
dc.title | Preliminary analysis of miRNA pathway in Schistosoma mansoni. | pt_BR |
dc.type | Artigo publicado em periodico | pt_BR |
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