Epitope mapping of the HSP83.1 protein of Leishmania braziliensis discloses novel targets for immunodiagnosis of tegumentary and visceral clinical forms of leishmaniasis.
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2014
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Souza, Daniel Menezes
Mendes, Tiago Antônio de Oliveira
Gomes, Matheus de Souza
Cunha, João Luís Reis
Nagem, Ronaldo Alves Pinto
Carneiro, Cláudia Martins
Coelho, Eduardo Antônio Ferraz
Galvão, Lúcia Maria da Cunha
Fujiwara, Ricardo Toshio
Bartholomeu, Daniella Castanheira
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Gold standard serological diagnostic methods focus on antigens that elicit a strong humoral immune response that is specific to
a certain pathogen. In this study, we used bioinformatics approaches to identify linear B-cell epitopes that are conserved among
Leishmania species but are divergent from the host species Homo sapiens and Canis familiaris and from Trypanosoma cruzi, the
parasite that causes Chagas disease, to select potential targets for the immunodiagnosis of leishmaniasis. Using these criteria, we
selected heat shock protein 83.1 of Leishmania braziliensis for this study. We predicted three linear B-cell epitopes in its sequence.
These peptides and the recombinant heat shock protein 83.1 (rHSP83.1) were tested in enzyme-linked immunosorbent
assays (ELISAs) against serum samples from patients with tegumentary leishmaniasis (TL) and visceral leishmaniasis (VL) and
from dogs infected with Leishmania infantum (canine VL [CVL]). Our data show that rHSP83.1 is a promising target in the diagnosis
of TL. We also identified specific epitopes derived from HSP83.1 that can be used in the diagnosis of human TL (peptide 3),
both human and canine VL (peptides 1 and 3), and all TL, VL, and CVL clinical manifestations (peptide 3). Receiver operating
characteristic (ROC) curves confirmed the superior performance of rHSP83.1 and peptides 1 and 3 compared to that of the soluble
L. braziliensis antigen and the reference test kit for the diagnosis of CVL in Brazil (EIE-LVC kit; Bio-Manguinhos, Fiocruz).
Our study thus provides proof-of-principle evidence of the feasibility of using bioinformatics to identify novel targets for the
immunodiagnosis of parasitic diseases using proteins that are highly conserved throughout evolution.
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SOUZA, D. M. et al. Epitope mapping of the HSP83.1 protein of Leishmania braziliensis discloses novel targets for immunodiagnosis of tegumentary and visceral clinical forms of leishmaniasis. Clinical and Vaccine Immunology, v. 21, n. 7, p. 949-959, jul. 2014. Disponível em: <http://cvi.asm.org/content/21/7/949>. Acesso em: 10 jan. 2017.