Analysis of the cytokine profile in spleen cells from dogs naturally infected by Leishmania chagasi.
dc.contributor.author | Lage, Ramon Silva | |
dc.contributor.author | Oliveira, Guilherme Corrêa de | |
dc.contributor.author | Busek, Solange Cristina Uber | |
dc.contributor.author | Guerra, Luanda Liboreiro | |
dc.contributor.author | Giunchetti, Rodolfo Cordeiro | |
dc.contributor.author | Oliveira, Rodrigo Corrêa de | |
dc.contributor.author | Reis, Alexandre Barbosa | |
dc.date.accessioned | 2012-07-10T13:15:56Z | |
dc.date.available | 2012-07-10T13:15:56Z | |
dc.date.issued | 2007 | |
dc.description.abstract | Recent studies suggest that asymptomatic dogs infected with canine visceral leishmaniasis (CVL) develop a Th1 immunological profile whilst oligosymptomatic and symptomatic CVL-infected animals present a Th2 profile. In the present study, an RT-PCR method has been standardised and employed to evaluate the frequency and the semi-quantitative level of expression of the cytokines IL-4, IL-10, IL-12, INF- g and TNF- a in splenocytes of 30 dogs naturally infected with Leishmania chagasiand of 7 non-infected dogs (NID). An increase in the level of expression of IL-12 ( p = 0.059) was detected in all CVL-infected dogs compared with NID. In dogs exhibiting high parasitism, the frequency of expression of IL-10 was higher ( p = 0.011) than in animals presenting low parasitism or medium parasitism (MP) and in NID animals, whilst the level of expression of IL-10 was higher (p = 0.0094) than in animals exhibiting MP and in the NID group. Positive correlations between the levels of expression of IL-10 with respect to the progression of the disease (IL-10: r = 0.3510; p = 0.0337) and the levels of expression of IL-10 and INF- g increase in parasitism (IL-10: r = 0.3428; p = 0.0438 and INF- g: r = 0.4690; p = 0.0045) were observed. Such data suggest that CVL is marked by a balanced production of Th1 and Th2 cytokines, with a predominant accumulation of IL-10 as a consequence of an increase in parasitic load and progression of the disease, and INF- g was related with the increase in parasitic load. | pt_BR |
dc.identifier.citation | LAGE, R. S. et al. Analysis of the cytokine profile in spleen cells from dogs naturally infected by Leishmania chagasi. Veterinary Immunology and immunopathology, v. 115, n. 1-2, p. 135-145, jan. 2007. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0165242706002741>. Acesso em: 10 jul. 2012. | pt_BR |
dc.identifier.issn | 01652427 | |
dc.identifier.uri | http://www.repositorio.ufop.br/handle/123456789/1027 | |
dc.language.iso | en_US | pt_BR |
dc.rights.license | O periódico Veterinary Immunology and Immunopathology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3303250782673. | |
dc.subject | Cytokines | pt_BR |
dc.subject | Canine disease | pt_BR |
dc.subject | Visceral leishmaniasis | pt_BR |
dc.subject | Leishmania chagasi | pt_BR |
dc.title | Analysis of the cytokine profile in spleen cells from dogs naturally infected by Leishmania chagasi. | pt_BR |
dc.type | Artigo publicado em periodico | pt_BR |