Yeast genes YOL002C and Sul1 are involved in neomycine resistance.
dc.contributor.author | Castro, Ieso de Miranda | |
dc.contributor.author | Cabral, Daniela Bastos | |
dc.contributor.author | Trópia, Maria José Magalhães | |
dc.contributor.author | Barnabé, Fabiana Maria de Almeida | |
dc.contributor.author | Brandão, Rogélio Lopes | |
dc.date.accessioned | 2017-03-20T12:50:26Z | |
dc.date.available | 2017-03-20T12:50:26Z | |
dc.date.issued | 2001 | |
dc.description.abstract | In previous studies we suggested the importance of the control of plasma membrane H+-ATPase by a phosphatidylinositol-like pathway for cellular proton extrusion in Saccharomyces cerevisiae (Brandão et al. 1994; Coccetti et al. 1998). The observations that provided the model above include the inhibition of the glucose-induced activation of the plasma membrane H+-ATPase as well as the inhibition of the glucose-induced external acidification by neomycin, a known inhibitor of the phosphatidylinositol turnover in eukaryotic cells. In this work, using two libraries, we isolated two yeast clones that were able to prevent the inhibition of glucose-induced activation of the H+-ATPase by neomycin. We show that the YOL002C gene, which encodes a protein of unknown function, and the SUL1 gene, which is a sulphate transporter belonging to the major facilitator superfamily, suppress growth inhibition by neomycin. However, they are not required for glucose-induced activation of the plasma membrane H+-ATPase. The resistance of the clones to neomycin is probably related to the level and/or activity of proteins functioning as drug extrusion pumps. | pt_BR |
dc.identifier.citation | CASTRO, I. de M. et al. Yeast genes YOL002C and Sul1 are involved in neomycine resistance. World Journal Of Microbiology And Biotechnology, v. 17, n. 4, p.399-402, 2001. Disponível em: <http://link.springer.com/article/10.1023/A:1016707627056>. Acesso em: 10 jan. 2017. | pt_BR |
dc.identifier.doi | https://doi.org/10.1023/A:1016707627056 | |
dc.identifier.issn | 1573-0972 | |
dc.identifier.uri | http://www.repositorio.ufop.br/handle/123456789/7393 | |
dc.identifier.uri2 | http://link.springer.com/article/10.1023/A:1016707627056 | pt_BR |
dc.language.iso | en_US | pt_BR |
dc.rights | restrito | pt_BR |
dc.subject | Growth inhibition | pt_BR |
dc.title | Yeast genes YOL002C and Sul1 are involved in neomycine resistance. | pt_BR |
dc.type | Artigo publicado em periodico | pt_BR |