Acarbose presents in vitro and in vivo antileishmanial activity against Leishmania infantum and is a promising therapeutic candidate against visceral leishmaniasis.
dc.contributor.author | Costa, Rafaella Rodrigues | |
dc.contributor.author | Silva, João Augusto Oliveira da | |
dc.contributor.author | Reis, Thiago Alves Rosa dos | |
dc.contributor.author | Tavares, Grasiele de Sousa Vieira | |
dc.contributor.author | Mendonça, Débora Vasconcelos Costa | |
dc.contributor.author | Freitas, Camila Simões de | |
dc.contributor.author | Lage, Daniela Pagliara | |
dc.contributor.author | Martins, Vívian Tamietti | |
dc.contributor.author | Antinarelli, Luciana Maria Ribeiro | |
dc.contributor.author | Machado, Amanda Sanchez | |
dc.contributor.author | Bandeira, Raquel Soares | |
dc.contributor.author | Ribeiro, Fernanda Ludolf | |
dc.contributor.author | Santos, Thaís Teodoro de Oliveira | |
dc.contributor.author | Brito, Rory Cristiane Fortes de | |
dc.contributor.author | Humbert, Maria Victoria | |
dc.contributor.author | Souza, Daniel Menezes | |
dc.contributor.author | Duarte, Mariana Costa | |
dc.contributor.author | Chávez Fumagalli, Miguel Angel | |
dc.contributor.author | Roatt, Bruno Mendes | |
dc.contributor.author | Coimbra, Elaine Soares | |
dc.contributor.author | Coelho, Eduardo Antônio Ferraz | |
dc.date.accessioned | 2021-09-30T16:58:46Z | |
dc.date.available | 2021-09-30T16:58:46Z | |
dc.date.issued | 2020 | pt_BR |
dc.description.abstract | Treatment against visceral leishmaniasis (VL) is mainly hampered by drug toxicity, long treatment regimens and/or high costs. Thus, the identifcation of novel and low-cost antileishmanial agents is urgent. Acarbose (ACA) is a specifc inhibitor of glucosidase-like proteins, which has been used for treating diabetes. In the present study, we show that this molecule also presents in vitro and in vivo specifc antileishmanial activity against Leishmania infantum. Results showed an in vitro direct action against L. infantum promastigotes and amastigotes, and low toxicity to mammalian cells. In addition, in vivo experiments performed using free ACA or incorporated in a Pluronic® F127-based polymeric micelle system called ACA/ Mic proved efective for the treatment of L. infantum-infected BALB/c mice. Treated animals presented signifcant reductions in the parasite load in their spleens, livers, bone marrows and draining lymph nodes when compared to the controls, as well as the development of antileishmanial Th1-type humoral and cellular responses based on high levels of IFN-γ, IL-12, TNF-α, GM-CSF, nitrite and IgG2a isotype antibodies. In addition, ACA or ACA-treated animals sufered from low organ toxicity. Treatment with ACA/Mic outperformed treatments using either Miltefosine or free ACA based on parasitological and immunological evaluations performed one and 15 days post-therapy. In conclusion, data suggest that the ACA/Mic is a potential therapeutic agent against L. infantum and merits further consideration for VL treatment. | pt_BR |
dc.identifier.citation | COSTA, R. R. et al. Acarbose presents in vitro and in vivo antileishmanial activity against Leishmania infantum and is a promising therapeutic candidate against visceral leishmaniasis. Medical Microbiology and Immunology, v. 210, p. 133-147, abr. 2021. Disponível em: <https://link.springer.com/article/10.1007%2Fs00430-021-00707-4>. Acesso em: 10 jun. 2021. | pt_BR |
dc.identifier.doi | https://doi.org/10.1007/s00430-021-00707-4 | pt_BR |
dc.identifier.issn | 1432-1831 | |
dc.identifier.uri | http://www.repositorio.ufop.br/jspui/handle/123456789/13833 | |
dc.identifier.uri2 | https://link.springer.com/article/10.1007%2Fs00430-021-00707-4 | pt_BR |
dc.language.iso | en_US | pt_BR |
dc.rights | restrito | pt_BR |
dc.subject | Treatment | pt_BR |
dc.subject | Drug repositioning | pt_BR |
dc.subject | Miltefosine | pt_BR |
dc.title | Acarbose presents in vitro and in vivo antileishmanial activity against Leishmania infantum and is a promising therapeutic candidate against visceral leishmaniasis. | pt_BR |
dc.type | Artigo publicado em periodico | pt_BR |
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