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Title: Histopathology, parasite density and cell phenotypes of the popliteal lymph node in canine visceral leishmaniasis.
Authors: Giunchetti, Rodolfo Cordeiro
Martins Filho, Olindo Assis
Carneiro, Cláudia Martins
Mayrink, Wilson
Marques, Marcos José
Tafuri, Wagner Luiz
Oliveira, Rodrigo Corrêa de
Reis, Alexandre Barbosa
Keywords: Canine visceral leishmaniasis
Leishmania chagasi
Lymph node
Lymphocyte subsets
Flow cytometry
Issue Date: 2008
Citation: GIUNCHETTI, R. C. et al. Histopathology, parasite density and cell phenotypes of the popliteal lymph node in canine visceral leishmaniasis. Veterinary Immunology and Immunopathology, v. 121, n. 1-2, p. 23-33, jan. 2008. Disponível em: <>. Acesso em: 05 jul. 2012.
Abstract: While enlargement of popliteal lymph nodes (LN) is frequently described in canine visceral leishmaniasis (CVL), there are few histopathologic studies of lymph nodes during this chronic immunopathological condition. Besides a detailed histopathologic analysis, we have characterized the parasite load and major immunophenotypic features of the LN inLeishmania (Leishmania ) chagasi-infected dogs. Our major histopathological findings highlight that hypertrophy/hyperplasia of LN cortical and medullary zones was the principal characteristic observed in asymptomatic dogs (AD), whereas atrophy of LN cortical zone was predominant in symptomatic animals (SD). The LN parasite density detected by anti- Leishmania immunohistochemical assay or expressed as Leishman Donovan Units was also highly correlated with the skin parasitism, the most reliable parameter to decode the clinical status of CVL. The major LN immunophenotypic changes during ongoing CVL were an increased frequency of T-lymphocytes, particularly CD8 + T-cells, up-regulation of MHC-II expression by lymphocytes and decreased levels of CD21 + B-cells. Our findings further demonstrated that changes in the LN B-lymphocyte compartment exhibited a negative correlation with the skin parasite load. Conversely, we also showed evidence for a positive association between skin parasitism and LN T-cell-mediated immunity, suggesting that T-cells, especially CD8 + lymphocytes, may have a Type-2 immunological profile in this lymphoid tissue in response to CVL.
ISSN: 01652427
metadata.dc.rights.license: O periódico Veterinary Immunology and Immunopathology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licpnça: 3281420862355.
Appears in Collections:DEACL - Artigos publicados em periódicos

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