Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/9233
Title: Uncovering Notch pathway in the parasitic flatworm Schistosoma mansoni.
Authors: Magalhães, Lizandra Guidi
Morais, Enyara Rezende
Machado, Carla Botelho
Gomes, Matheus de Souza
Cabral, Fernanda Janku
Souza, Júlia Medeiros
Soares, Cláudia Sossai
Cota, Renata Guerra de Sá
Borges, William de Castro
Rodrigues, Vanderlei
Keywords: Gamma-secretase complex
Issue Date: 2016
Citation: MAGALHÃES, L. G. et al. Uncovering Notch pathway in the parasitic flatworm Schistosoma mansoni. Parasitology Research, v. 115, p. 3951-3961, 2016. Disponível em: <https://link.springer.com/article/10.1007/s00436-016-5161-8>. Acesso em: 15 set. 2017.
Abstract: Several signaling molecules that govern development in higher animals have been identified in the parasite Schistosoma mansoni, including the transforming growth factor β, protein tyrosine kinases, nuclear hormone receptors, among others. The Notch pathway is a highly conserved signaling mechanism which is involved in a wide variety of developmental processes including embryogenesis and oogenesis in worms and flies. Here we aimed to provide the molecular reconstitution of the Notch pathway in S. mansoni using the available transcriptome and genome databases. Our results also revealed the presence of the transcripts coded for SmNotch, SmSu(H), SmHes, and the gamma-secretase complex (SmNicastrin, SmAph-1, and SmPen-2), throughout all the life stages analyzed. Besides, it was observed that the viability and separation of adult worm pairs were not affected by treatment with N-[N(3,5)-difluorophenacetyl)-L-Alanyl]- S-phenylglycine t-butyl ester (DAPT), a Notch pathway inhibitor. Moreover, DAPT treatment decreased the production of phenotypically normal eggs and arrested their development in culture. Our results also showed a significant decrease in SmHes transcript levels in both adult worms and eggs treated withDAPT. These results provide, for the first time, functional validation of the Notch pathway in S. mansoni and suggest its involvement in parasite oogenesis and embryogenesis. Given the complexity of the Notch pathway, further experiments shall highlight the full repertoire of Notch-mediated cellular processes throughout the S. mansoni life cycle.
URI: http://www.repositorio.ufop.br/handle/123456789/9233
metadata.dc.identifier.uri2: https://link.springer.com/article/10.1007/s00436-016-5161-8
ISSN: 14321955 
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