Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/9186
Title: Lycopene inhibits reactive oxygen species production in SK-Hep-1 cells and attenuates acetaminophen-induced liver injury in C57BL/6 mice.
Authors: Bandeira, Ana Carla Balthar
Silva, Talita Prato da
Araújo, Glaucy Rodrigues de
Araújo, Carolina Morais
Silva, Rafaella Cecília da
Lima, Wanderson Geraldo de
Bezerra, Frank Silva
Costa, Daniela Caldeira
Keywords: Hepatotoxicity
Oxidative stress
Issue Date: 2017
Citation: BANDEIRA, A. C. B. et al. Lycopene inhibits reactive oxygen species production in SK-Hep-1 cells and attenuates acetaminophen-induced liver injury in C57BL/6 mice. Chemico-Biological Interactions, v. 263, p. 7-17, 2017. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0009279716307219>. cesso em: 15 set. 2017
Abstract: Our aim was to investigate the antioxidant potential of lycopene in different experimental liver models: in vitro, to evaluate the influence of lycopene on reactive oxygen species (ROS) production mediated by the PKC pathway and in vivo, to evaluate the protective effects of lycopene in an experimental model of hepatotoxicity. The in vitro study assessed the lycopene antioxidant potential by the quantification of ROS production in SK-Hep-1 cells unstimulated or stimulated by an activator of the PKC pathway. The role of NADPH oxidase was evaluated by measuring its inhibition potential using an inhibitor of this enzyme. In the in vivo study, male C57BL/6 mice received lycopene (10 or 100 mg/kg by oral gavage) and 1 h later, acetaminophen (APAP) (500 mg/kg) was administrated. Lycopene decreased ROS production in SK-Hep- 1 cells through inhibition of NADPH oxidase, brought about in the PKC pathway. Lycopene improved hepatotoxicity acting as an antioxidant, reduced GSSG and regulated tGSH and CAT levels, reduced oxidative damage primarily by decreasing protein carbonylation, promoted the downregulation of MMP- 2 and reduced areas of necrosis improving the general appearance of the lesion in C57BL/6 mice. Lycopene is a natural compound that was able to inhibit the production of ROS in vitro and mitigate the damage caused by APAP overdose in vivo.
URI: http://www.repositorio.ufop.br/handle/123456789/9186
metadata.dc.identifier.uri2: http://www.sciencedirect.com/science/article/pii/S0009279716307219
ISSN: 00092797
Appears in Collections:DECBI - Artigos publicados em periódicos

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