Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/9185
Title: Baccharis trimera inhibits reactive oxygen species production through PKC and down-regulation p47phox phosphorylation of NADPH oxidase in SK Hep-1 cells.
Authors: Araújo, Glaucy Rodrigues de
Rabelo, Ana Carolina Silveira
Meira, Janaína Serenato
Rossoni Júnior, Joamyr Victor
Borges, William de Castro
Cota, Renata Guerra de Sá
Batista, Maurício Azevedo
Lemos, Denise da Silveira
Souza, Gustavo Henrique Bianco de
Brandão, Geraldo Célio
Chaves, Míriam Martins
Costa, Daniela Caldeira
Keywords: Nicotinamide adenine dinucleotide phosphate oxidase
Reactive oxygen species
Quercetin
Rutin
Issue Date: 2017
Citation: ARAÚJO, G. R. et al. Baccharis trimera inhibits reactive oxygen species production through PKC and down-regulation p47phox phosphorylation of NADPH oxidase in SK Hep-1 cells. Experimental Biology and Medicine, v. 242, p. 333-343, 2017. Disponível em: <http://journals.sagepub.com/doi/pdf/10.1177/1535370216672749>. Acesso em: 15 set. 2017.
Abstract: Baccharis trimera, popularly known as ‘‘carqueja’’, is a native South-American plant possessing a high concentration of polyphenolic compounds and therefore high antioxidant potential. Despite the antioxidant potential described for B. trimera, there are no reports concerning the signaling pathways involved in this process. So, the aim of the present study was to assess the influence of B. trimera on the modulation of PKC signaling pathway and to characterize the effect of the nicotinamide adenine dinucleotide phosphate oxidase enzyme (NOX) on the generation of reactive oxygen species in SK Hep-1 cells. SK-Hep 1 cells were treated with B. trimera, quercetin, or rutin and then stimulated or notwith PMA/ionomycin and labeled with carboxy H2DCFDA for detection of reactive oxygen species by flow cytometer. The PKC expression by Western blot and enzyme activity was performed to evaluate the influence of B. trimera and quercetin on PKC signaling pathway. p47phox and p47phox phosphorylated expression was performed byWestern blot to evaluate the influence of B. trimera on p47phox phosphorylation. The results showed that cells stimulated with PMA/ionomycin (activators of PKC) showed significantly increased reactive oxygen species production, and this production returned to baseline levels after treatment with DPI (NOX inhibitor). Both B. trimera and quercetin modulated reactive oxygen species production through the inhibition of PKC protein expression and enzymatic activity, also with inhibition of p47phox phosphorylation. Taken together, these results suggest that B. trimera has a potentialmechanism for inhibiting reactive oxygen species production through the PKC signaling pathway and inhibition subunit p47phox phosphorylation of nicotinamide adenine dinucleotide phosphate oxidase.
URI: http://www.repositorio.ufop.br/handle/123456789/9185
metadata.dc.identifier.uri2: http://journals.sagepub.com/doi/pdf/10.1177/1535370216672749
metadata.dc.identifier.doi: https://doi.org/10.1177%2F1535370216672749
ISSN: 1535-3699 
Appears in Collections:DECBI - Artigos publicados em periódicos

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