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http://www.repositorio.ufop.br/jspui/handle/123456789/9027
Título : | Acyl homoserine lactone changes the abundance of proteins and the levels of organic acids associated with stationary phase in Salmonella Enteritidis. |
Autor : | Almeida, Felipe Alves de Pimentel Filho, Natan de Jesus Carrijo, Lanna Clícia Bento, Cláudia Braga Pereira Pereira, Maria Cristina Baracat Pinto, Uelinton Manoel Oliveira, Leandro Licursi de Vanetti, Maria Cristina Dantas |
Palabras clave : | Autoinducer Differentially abundant proteins Formic acid Starvation Stationary phase |
Fecha de publicación : | 2017 |
Citación : | ALMEIDA, F. A. de et al. Acyl homoserine lactone changes the abundance of proteins and the levels of organic acids associated with stationary phase in Salmonella Enteritidis. Microbial Pathogenesis, v. 102, p. 148-159, 2017. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0882401016304491>. Acesso em: 29 ago. 2017. |
Resumen : | Quorum sensing (QS) is cell-cell communication mechanism mediated by signaling molecules known as autoinducers (AIs) that lead to differential gene expression. Salmonella is unable to synthesize the AI-1 acyl homoserine lactone (AHL), but is able to recognize AHLs produced by other microorganisms through SdiA protein. Our study aimed to evaluate the influence of AI-1 on the abundance of proteins and the levels of organic acids of Salmonella Enteritidis. The presence of N-dodecyl-homoserine lactone (C12- HSL) did not interfere on the growth or the total amount of extracted proteins of Salmonella. However, the abundance of the proteins PheT, HtpG, PtsI, Adi, TalB, PmgI (or GpmI), Eno, and PykF enhanced while the abundance of the proteins RplB, RplE, RpsB, Tsf, OmpA, OmpC, OmpD, and GapA decreased when Salmonella Enteritidis was anaerobically cultivated in the presence of C12-HSL. Additionally, the bacterium produced less succinic, lactic, and acetic acids in the presence of C12-HSL. However, the concentration of extracellular formic acid reached 20.46 mM after 24 h and was not detected when the growth was in the absence of AI-1. Considering the cultivation period for protein extraction, their abundance, process and function, as well as the levels of organic acids, we observed in cells cultivated in presence of C12-HSL a correlation with what is described in the literature as entry into the stationary phase of growth, mainly related to nitrogen and amino acid starvation and acid stress. Further studies are needed in order to determine the specific role of the differentially abundant proteins and extracellular organic acids secreted by Salmonella in the presence of quorum sensing signaling molecules. |
URI : | http://www.repositorio.ufop.br/handle/123456789/9027 |
metadata.dc.identifier.uri2: | http://www.sciencedirect.com/science/article/pii/S0882401016304491 |
metadata.dc.identifier.doi: | https://doi.org/10.1016/j.micpath.2016.11.027 |
ISSN : | 0882-4010 |
Aparece en las colecciones: | DEALI - Artigos publicados em periódicos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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ARTIGO_AcylHomoserineLactone.pdf Restricted Access | 1,59 MB | Adobe PDF | Visualizar/Abrir |
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