Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/jspui/handle/123456789/9027
Title: Acyl homoserine lactone changes the abundance of proteins and the levels of organic acids associated with stationary phase in Salmonella Enteritidis.
Authors: Almeida, Felipe Alves de
Pimentel Filho, Natan de Jesus
Carrijo, Lanna Clícia
Bento, Cláudia Braga Pereira
Pereira, Maria Cristina Baracat
Pinto, Uelinton Manoel
Oliveira, Leandro Licursi de
Vanetti, Maria Cristina Dantas
Keywords: Autoinducer
Differentially abundant proteins
Formic acid
Starvation
Stationary phase
Issue Date: 2017
Citation: ALMEIDA, F. A. de et al. Acyl homoserine lactone changes the abundance of proteins and the levels of organic acids associated with stationary phase in Salmonella Enteritidis. Microbial Pathogenesis, v. 102, p. 148-159, 2017. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0882401016304491>. Acesso em: 29 ago. 2017.
Abstract: Quorum sensing (QS) is cell-cell communication mechanism mediated by signaling molecules known as autoinducers (AIs) that lead to differential gene expression. Salmonella is unable to synthesize the AI-1 acyl homoserine lactone (AHL), but is able to recognize AHLs produced by other microorganisms through SdiA protein. Our study aimed to evaluate the influence of AI-1 on the abundance of proteins and the levels of organic acids of Salmonella Enteritidis. The presence of N-dodecyl-homoserine lactone (C12- HSL) did not interfere on the growth or the total amount of extracted proteins of Salmonella. However, the abundance of the proteins PheT, HtpG, PtsI, Adi, TalB, PmgI (or GpmI), Eno, and PykF enhanced while the abundance of the proteins RplB, RplE, RpsB, Tsf, OmpA, OmpC, OmpD, and GapA decreased when Salmonella Enteritidis was anaerobically cultivated in the presence of C12-HSL. Additionally, the bacterium produced less succinic, lactic, and acetic acids in the presence of C12-HSL. However, the concentration of extracellular formic acid reached 20.46 mM after 24 h and was not detected when the growth was in the absence of AI-1. Considering the cultivation period for protein extraction, their abundance, process and function, as well as the levels of organic acids, we observed in cells cultivated in presence of C12-HSL a correlation with what is described in the literature as entry into the stationary phase of growth, mainly related to nitrogen and amino acid starvation and acid stress. Further studies are needed in order to determine the specific role of the differentially abundant proteins and extracellular organic acids secreted by Salmonella in the presence of quorum sensing signaling molecules.
URI: http://www.repositorio.ufop.br/handle/123456789/9027
metadata.dc.identifier.uri2: http://www.sciencedirect.com/science/article/pii/S0882401016304491
metadata.dc.identifier.doi: https://doi.org/10.1016/j.micpath.2016.11.027
ISSN: 0882-4010
Appears in Collections:DEALI - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
ARTIGO_AcylHomoserineLactone.pdf
  Restricted Access
1,59 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.