Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/8899
Title: Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs using peptides selected from hypothetical proteins identified by an immunoproteomic approach.
Authors: Chávez Fumagalli, Miguel Angel
Martins, Vívian Tamietti
Testasicca, Miriam Conceição de Souza
Lage, Daniela Pagliara
Costa, Lourena Emanuele
Lage, Paula Souza
Duarte, Mariana Costa
Ker, Henrique Gama
Ribeiro, Tatiana Gomes
Carvalho, Fernando A. A.
Régis, Wiliam César Bento
Reis, Alexandre Barbosa
Tavares, Carlos Alberto Pereira
Soto, Manuel
Fernandes, Ana Paula
Coelho, Eduardo Antônio Ferraz
Issue Date: 2013
Citation: CHÁVEZ FUMAGALLI, M. A. et al. Sensitive and specific serodiagnosis of Leishmania infantum infection in dogs using peptides selected from hypothetical proteins identified by an immunoproteomic approach. Clinical and Vaccine Immunology, v. 20, p. 835-841, 2013. Disponível em: <http://cvi.asm.org/content/20/6/835.figures-only>. Acesso em: 10 nov. 2016.
Abstract: In Brazil, the percentage of infected dogs living in areas where canine visceral leishmaniasis (CVL) is endemic ranges from 10 to 62%; however, the prevalence of infection in dogs is probably higher than figures reported from serological studies. In addition, problems with the occurrence of false-positive or false-negative results in the serodiagnosis of CVL have been reported. The present work analyzed the potential of synthetic peptides mapped from hypothetical proteins for improvement of the serodiagnosis of Leishmania infantum infection in dogs. From 26 identified leishmanial proteins, eight were selected, considering that no homologies between these proteins and others from trypanosomatide sequence databases were encountered. The sequences of these proteins were mapped to identify linear B-cell epitopes, and 17 peptides were synthesized and tested in enzyme-linked immunosorbent assays (ELISAs) for the serodiagnosis of L. infantum infection in dogs. Of these, three exhibited sensitivity and specificity values higher than 75% and 90%, respectively, to differentiate L. infantum-infected animals from Trypanosoma cruziinfected animals and healthy animals. Soluble Leishmania antigen (SLA) showed poor sensitivity (4%) and specificity (36%) to differentiate L. infantum-infected dogs from healthy and T. cruzi-infected dogs. Lastly, the three selected peptides were combined in different mixtures and higher sensitivity and specificity values were obtained, even when sera from T. cruzi-infected dogs were used. The study’s findings suggest that these three peptides can constitute a potential tool for more sensitive and specific serodiagnosis of L. infantum infection in dogs.
URI: http://www.repositorio.ufop.br/handle/123456789/8899
ISSN: 1556679X
metadata.dc.rights.license: O periódico Clinical and Vaccine Immunology permite o arquivamento da versão PDF do editor. Fonte: Sherpa/Romeo <http://www.sherpa.ac.uk/romeo/issn/1556-6811/>. Acesso em: 08 ago. 2017.
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