Please use this identifier to cite or link to this item:
|Title:||Identification of a novel agonist-like autoantibody in preeclamptic patients.|
|Authors:||Velloso, Elizabeth Portugal|
Pimentel, Renata Lúcia
Braga, Janaína F.
Cabral, Antônio Carlos Vieira
Reis, Zilma Silveira Nogueira
Santos, Robson Augusto Souza dos
|Citation:||VELLOSO, E. P. et al. Identification of a novel agonist-like autoantibody in preeclamptic patients. American Journal of Hypertension, v. 29, p. 405-412, 2016. Disponível em: <https://academic.oup.com/ajh/article-lookup/doi/10.1093/ajh/hpv099>. Acesso em: 29 ago. 2017.|
|Abstract:||BACKGROUND Recent studies have shown that preeclampsia (PE) is associated with the presence of autoantibodies (AABs) that activate the angiotensin II AT1 receptor, which could contribute to many of the symptoms of PE. METHODS To investigate the frequency and the targets of AABs in preeclamptic women (31 cases) and healthy pregnant normotensive women (29 cases) in Brazil, antibodies from serum samples were detected by a bioassay using spontaneously beating neonatal rat cardiomyocytes in culture. In the cardiomyocytes, the agonistic AABs induce a positive or negative chronotropic response, mimicking the corresponding receptor agonists. The specificity of the AAB response was identified by specific receptor antagonists. RESULTS Thirty preeclamptic patients (97%) presented AABs against the angiotensin II AT1 receptor. The agonistic effect of the AAB was blocked by irbesartan and neutralized by a peptide corresponding to the second extracellular loop of this receptor. Strikingly, we discovered that all sera from the severe preeclamptic patients (16 cases) contained a novel agonist- like AAB directed against the endothelin-1 ETA receptor in addition to the AABs against the angiotensin II AT1 receptor. This AAB was selectively blocked by the antagonist BQ-123, antagonized by the protein kinase C (PKC) inhibitor Calphostin C and neutralized by peptides corresponding to the second extracellular loop of the endothelin-1 ETA receptor subtype. CONCLUSIONS We described, for the first time, the presence of endothelin-1 ETA receptor AABs in PE. Our results suggest that the presence of both agonistic AABs may be involved in the pathogenesis of severe PE.|
|Appears in Collections:||DECGP - Artigos publicados em periódicos|
Files in This Item:
|ARTIGO_IdentificationNovelAgonist.pdf||895,05 kB||Adobe PDF||View/Open Request a copy|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.