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Title: Molecular characterization of early colonizer bacteria from wastes in a steel plant.
Authors: Freitas, Dulcecleide Bezerra de
Bittencourt, Cláudia Iracema Lima
Guimarães, Mariana de Paula Reis
Silva, Patricia Costa Lima da
Assis, Paulo Santos
Souza, Edmar Chartone de
Nascimento, Andrea Maria Amaral
Keywords: Box fingerprinting
Steelmaking waste
Heavy metal
Issue Date: 2008
Citation: FREITAS, D. B. de et al. Molecular characterization of early colonizer bacteria from wastes in a steel plant. Letters in Applied Microbiology, v. 47, p. 241-249, 2008. Disponível em: <>. Acesso em: 20 jul. 2017.
Abstract: Forty-nine bacteria isolated from four newly-produced waste samples of a steel industry, which had a high content of CaO, MgO, Cr and P2O5, were characterized molecularly and phenotypically by susceptibility testing against heavy metals. Methods and Results: Phylogenetic analysis using 16S rRNA gene sequences revealed that the isolates belonged to nine genera, Pseudomonas, Micrococcus, Acinetobacter, Bacillus, Dietzia, Kocuria, Diaphorobacter, Staphylococcus and Brevibacillus. Besides, some isolates could be affiliated to species: M. luteus, Ac. junii, Ac. schindleri, B. cereus, K. marina, D. nitroreducens and Staph. warneri. The bacteria that were characterized are taxonomically diverse, and Pseudomonas and Micrococcus predominated. Fingerprinting BOX-PCR revealed high genomic heterogeneity among the isolates. Among the heavy metal compounds Zn, Ni, Pb and Cu were least toxic to the bacterial isolates, whereas Ag inhibited all isolates at 0Æ001 mmol l)1.
ISSN: 1472-765X
Appears in Collections:DEMET - Artigos publicados em periódicos

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