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dc.contributor.authorFerreira, Rafael Marini-
dc.contributor.authorOliveira, Amanda Carolina Paulino de-
dc.contributor.authorMoreira, Leandro Marcio-
dc.contributor.authorBelasque Junior, José-
dc.contributor.authorGourbeyre, Edith-
dc.contributor.authorSiguier, Patricia-
dc.contributor.authorFerro, Maria Inês Tiraboschi-
dc.contributor.authorFerro, Jesus Aparecido-
dc.contributor.authorChandler, Michael-
dc.contributor.authorVarani, Alessandro de Mello-
dc.identifier.citationFERREIRA, R. M. et al. A TALE of transposition: Tn3-like transposons play a major role in the spread of pathogenicity determinants of Xanthomonas citri and other Xanthomonads. mBio, v. 6, p. e02505-14, 2015. Disponível em: <>. Acesso em: 05 ago. 2017.pt_BR
dc.description.abstractMembers of the genus Xanthomonas are among the most important phytopathogens. A key feature of Xanthomonas pathogenesis is the translocation of type III secretion system (T3SS) effector proteins (T3SEs) into the plant target cells via a T3SS. Several T3SEs and a murein lytic transglycosylase gene (mlt, required for citrus canker symptoms) are found associated with three transposition-related genes in Xanthomonas citri plasmid pXAC64. These are flanked by short inverted repeats (IRs). The region was identified as a transposon, TnXax1, with typical Tn3 family features, including a transposase and two recombination genes. Two 14-bp palindromic sequences within a 193-bp potential resolution site occur between the recombination genes. Additional derivatives carrying different T3SEs and other passenger genes occur in different Xanthomonas species. The T3SEs include transcription activator-like effectors (TALEs). Certain TALEs are flanked by the same IRs as found in TnXax1 to form mobile insertion cassettes (MICs), suggesting that they may be transmitted horizontally. A significant number of MICs carrying other passenger genes (including a number of TALE genes) were also identified, flanked by the same TnXax1 IRs and delimited by 5-bp target site duplications. We conclude that a large fraction of T3SEs, including individual TALEs and potential pathogenicity determinants, have spread by transposition and that TnXax1, which exhibits all of the essential characteristics of a functional transposon, may be involved in driving MIC transposition. We also propose that TALE genes may diversify by fork slippage during the replicative Tn3 family transposition. These mechanisms may play a crucial role in the emergence of Xanthomonas pathogenicity.pt_BR
dc.titleA TALE of transposition : Tn3-like transposons play a major role in the spread of pathogenicity determinants of Xanthomonas citri and other Xanthomonads.pt_BR
dc.typeArtigo publicado em periodicopt_BR
dc.rights.licenseThis is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited. Fonte: O próprio artigo.pt_BR
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