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Title: Inhibitory efects of 7-epiclusianone on glucan synthesis, acidogenicityand biofilm formation by Streptococcus mutans.
Authors: Murata, Ramiro Mendonça
Almeida, Luciana Salles Branco de
Yatsuda, Regiane
Santos, Marcelo Henrique dos
Nagem, Tanus Jorge
Rosalen, Pedro Luiz
Koo, Hyun
Keywords: 7-epiclusianone
Streptococcus mutans
Issue Date: 2008
Citation: MURATA, R. M. et al. Inhibitory efects of 7-epiclusianone on glucan synthesis, acidogenicityand biofilm formation by Streptococcus mutans. FEMS Microbiology Letters, v. 282, p. 174-181, 2008. Disponível em: <>. Acesso em: 20 mai. 2017.
Abstract: The aim of this study was to examine the effects of 7-epiclusianone, a new prenylated benzophenone isolated from the plant Rheedia gardneriana, on some of the virulence properties of Streptococcus mutans associated with biofilm development and acidogenicity. The synthesis of glucans by glucosyltransferases B (GTF B) and C (GTF C) was markedly reduced by 7-epiclusianone showing more than 80% inhibition of enzymatic activity at a concentration of 100 mgmL 1. Doublereciprocal analysis (Lineweaver–Burk plots) revealed that the inhibition of GTF B activity was noncompetitive (mixed) while GTF C was inhibited uncompetitively. The glycolytic pH drop by S. mutans cells was also disrupted by 7-epiclusianone without affecting the bacterial viability, an effect that can be attributed, in part, to inhibition of F-ATPase activity (61.1 3.0% inhibition at 100 mgmL 1). Furthermore, topical applications (1-min exposure, twice daily) of 7-epiclusianone (at 250 mgmL 1) disrupted biofilm formation and physiology. The biomass (dryweight), extracellular insoluble polysaccharide concentration and acidogenicity of the biofilms were significantly reduced by the test agent (Po0.05). The data show that 7-epiclusianone disrupts the extracellular and intracellular sugar metabolism of S. mutans, and holds promise as a novel, naturally occurring compound to prevent biofilm-related oral diseases.
ISSN: 1574-6968
Appears in Collections:DEQUI - Artigos publicados em periódicos

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