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Título: | Highly conserved CDR3 region in circulating CD4(+) Vβ5(+) T cells may be associated with cytotoxic activity in Chagas disease. |
Autor(es): | Menezes, Cristiane Alves da Silva Sullivan, A. K. Falta, M. T. Mack, D. G. Freed, B. M. Rocha, Manoel Otávio da Costa Gollob, Kenneth John Fontenot, A. P. Dutra, Walderez Ornelas |
Data do documento: | 2012 |
Referência: | MENEZES, C. A. da S. et al. Highly conserved CDR3 region in circulating CD4(+) Vβ5(+) T cells may be associated with cytotoxic activity in Chagas disease. Clinical and Experimental Immunology, v. 169, p. 109-118, 2012. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2012.04608.x/abstract>. Acesso em: 19 fev. 2017. |
Resumo: | Human infection with Trypanosoma cruzi leads to Chagas disease, which presents as several different clinical conditions ranging from an asymptomatic form to a severe dilated cardiomyopathy. Several studies have demonstrated that T cells play a critical role in the development of cardiac pathology, as well as in immunoregulation during chronic disease. However, the mechanisms that drive protective or pathogenic T cell response are not known.We have shown that CD4+ T cells from chagasic patients preferentially express T cell receptor (TCR) b-chain variable region (Vb) 5. The aim of this work was to determine whether T cells expressing this particular Vb region displayed variable or restricted CDR3 sequences, as an indicator of the nature of the stimulus leading to the activation of these T cells in vivo. Additionally, we aimed to evaluate phenotypic characteristics of these cells that might be associated with pathology. CDR3 junctional region sequencing of Vb5·1 expressing CD4+ T cells revealed the occurrence of a highly homologous CDR3 region with conserved TCR Jb region usage among patients with cardiac, but not indeterminate, Chagas disease. Moreover, correlation analysis indicated that the frequency of CD4+Vb5·1+ cells is associated with granzyme A expression, suggesting that these cells might display cytotoxic function. Together these results provide new insight into T cell recognition of antigens involved in Chagas disease and suggest that these cells may be implicated in the pathogenesis of chagasic cardiomyopathy. |
URI: | http://www.repositorio.ufop.br/handle/123456789/7880 |
Link para o artigo: | http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2012.04608.x/abstract |
DOI: | https://doi.org/10.1111/j.1365-2249.2012.04608.x |
ISSN: | 1365-2249 |
Aparece nas coleções: | DECBI - Artigos publicados em periódicos |
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ARTIGO_HighlyConservedCDR3.pdf Restricted Access | 470,44 kB | Adobe PDF | Visualizar/Abrir |
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