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Title: LC UV methodology for simultaneous.
Authors: Souza, Jacqueline de
Kano, Eunice Kazue
Koono, Eunice Emiko Mori
Schramm, Simone Grigoleto
Porta, Valentina
Storpirtis, Sílvia
Keywords: Column liquid chromatography
Issue Date: 2008
Citation: SOUZA, J de et al. LC UV methodology for simultaneous. Chromatographia, Wiesbaden, v. 69, p. S231-S235, 2008. Disponível em: <> Acesso em: 10 jan. 2017
Abstract: This study describes an accurate, sensitive, and specific chromatographic method for the simultaneous quantitative determination of lamivudine and zidovudine in human blood plasma, using stavudine as an internal standard. The chromatographic separation was performed using a C8 column (150 9 4.6 mm, 5 lm), and ultraviolet absorbency detection at 270 nm with gradient elution. Two mobile phases were used. Phase A contained 10 mM potassium phosphate and 3% acetonitrile, whereas Phase B contained methanol. A linear gradient was used with a variability of A-B phase proportion from 98–2% to 72–28%, respectively. The drug extraction was performed with two 4 mL aliquots of ethyl acetate.
ISSN: 0009-5893
Appears in Collections:DEFAR - Artigos publicados em periódicos

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