Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/7394
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dc.contributor.authorBrandão, Rogélio Lopes-
dc.contributor.authorCastro, Ieso de Miranda-
dc.contributor.authorBambirra, Eduardo Alves-
dc.contributor.authorAmaral, Sheila Coutinho-
dc.contributor.authorFietto, Luciano Gomes-
dc.contributor.authorTrópia, Maria José Magalhães-
dc.contributor.authorNeves, Maria José-
dc.contributor.authorSantos, Raquel Gouvêa dos-
dc.contributor.authorGomes, Newton Carlos Marcial-
dc.contributor.authorNicoli, Jacques Robert-
dc.date.accessioned2017-03-20T12:55:44Z-
dc.date.available2017-03-20T12:55:44Z-
dc.date.issued1998-
dc.identifier.citationBRANDÃO, R. L. et al. Intracellular signal triggered by cholera toxin in Saccharomyces boulardii and Saccharomyces cerevisiae. Applied and Environmental Microbiology, v. 642, p. 564-568, 1998. Disponível em: <http://aem.asm.org/content/64/2/564.long> Acesso em: 10 jan. 2017pt_BR
dc.identifier.issn1098-5336-
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/7394-
dc.description.abstractAs is the case for Saccharomyces boulardii, Saccharomyces cerevisiae W303 protects Fisher rats against cholera toxin (CT). The addition of glucose or dinitrophenol to cells of S. boulardii grown on a nonfermentable carbon source activated trehalase in a manner similar to that observed for S. cerevisiae. The addition of CT to the same cells also resulted in trehalase activation. Experiments performed separately on the A and B subunits of CT showed that both are necessary for activation. Similarly, the addition of CT but not of its separate subunits led to a cyclic AMP (cAMP) signal in both S. boulardii and S. cerevisiae. These data suggest that trehalase stimulation by CT probably occurred through the cAMP-mediated protein phosphorylation cascade. The requirement of CT subunit B for both the cAMP signal and trehalase activation indicates the presence of a specific receptor on the yeasts able to bind to the toxin, a situation similar to that observed for mammalian cells. This hypothesis was reinforced by experiments with 125I-labeled CT showing specific binding of the toxin to yeast cells. The adhesion of CT to a receptor on the yeast surface through the B subunit and internalization of the A subunit (necessary for the cAMP signal and trehalase activation) could be one more mechanism explaining protection against the toxin observed for rats treated with yeasts.pt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.titleIntracellular signal triggered by cholera toxin in Saccharomyces boulardii and Saccharomyces cerevisiae.pt_BR
dc.typeArtigo publicado em periodicopt_BR
dc.identifier.uri2http://aem.asm.org/content/64/2/564.longpt_BR
Appears in Collections:DEFAR - Artigos publicados em periódicos

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