Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/7394
Title: Intracellular signal triggered by cholera toxin in Saccharomyces boulardii and Saccharomyces cerevisiae.
Authors: Brandão, Rogélio Lopes
Castro, Ieso de Miranda
Bambirra, Eduardo Alves
Amaral, Sheila Coutinho
Fietto, Luciano Gomes
Trópia, Maria José Magalhães
Neves, Maria José
Santos, Raquel Gouvêa dos
Gomes, Newton Carlos Marcial
Nicoli, Jacques Robert
Issue Date: 1998
Citation: BRANDÃO, R. L. et al. Intracellular signal triggered by cholera toxin in Saccharomyces boulardii and Saccharomyces cerevisiae. Applied and Environmental Microbiology, v. 642, p. 564-568, 1998. Disponível em: <http://aem.asm.org/content/64/2/564.long> Acesso em: 10 jan. 2017
Abstract: As is the case for Saccharomyces boulardii, Saccharomyces cerevisiae W303 protects Fisher rats against cholera toxin (CT). The addition of glucose or dinitrophenol to cells of S. boulardii grown on a nonfermentable carbon source activated trehalase in a manner similar to that observed for S. cerevisiae. The addition of CT to the same cells also resulted in trehalase activation. Experiments performed separately on the A and B subunits of CT showed that both are necessary for activation. Similarly, the addition of CT but not of its separate subunits led to a cyclic AMP (cAMP) signal in both S. boulardii and S. cerevisiae. These data suggest that trehalase stimulation by CT probably occurred through the cAMP-mediated protein phosphorylation cascade. The requirement of CT subunit B for both the cAMP signal and trehalase activation indicates the presence of a specific receptor on the yeasts able to bind to the toxin, a situation similar to that observed for mammalian cells. This hypothesis was reinforced by experiments with 125I-labeled CT showing specific binding of the toxin to yeast cells. The adhesion of CT to a receptor on the yeast surface through the B subunit and internalization of the A subunit (necessary for the cAMP signal and trehalase activation) could be one more mechanism explaining protection against the toxin observed for rats treated with yeasts.
URI: http://www.repositorio.ufop.br/handle/123456789/7394
metadata.dc.identifier.uri2: http://aem.asm.org/content/64/2/564.long
ISSN: 10985336
Appears in Collections:DEFAR - Artigos publicados em periódicos

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