Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/7263
Title: An antigenic domain within a catalytically active leishmania infantum nucleoside triphosphate diphosphohydrolase (NTPDase 1) is a target of inhibitory antibodies.
Authors: Maia, Ana Carolina Ribeiro Gomes
Porcino, Gabriane Nascimento
Detoni, Michelle de Lima
Emídio, Nayara Braga
Marconato, Danielle Gomes
Pinto, Priscila de Faria
Fessel, Melissa Regina
Reis, Alexandre Barbosa
Juliano Neto, Luiz
Juliano, Maria Aparecida
Marques, Marcos José
Vasconcelos, Eveline Gomes
Keywords: Potato apyrase
Conserved domain
Visceral leishmaniasis
Issue Date: 2013
Citation: MAIA, A. C. R. G. et al. An antigenic domain within a catalytically active leishmania infantum nucleoside triphosphate diphosphohydrolase (NTPDase 1) is a target of inhibitory antibodies. Parasitology International, v. 18, p.44-52, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S1383576912001274>. Acesso em: 10 out. 2016.
Abstract: Weidentified a shared B domainwithin nucleoside triphosphate diphosphohydrolases (NTPDases) of plants and parasites. Now, an NTPDase activity not affected by inhibitors of adenylate kinase and ATPases was detected in Leishmania infantum promastigotes. By non-denaturing gel electrophoresis of detergent-homogenized promastigote preparation, an active band hydrolyzing nucleosides di- and triphosphate was visualized and, following SDS-PAGE and silver staining was identified as a single polypeptide of 50 kDa. By Western blots, it was recognized by immune sera raised against potato apyrase (SA), r-pot B domain (SB), a recombinant polypeptide derived fromthe potato apyrase, and LbB1LJ (SC) or LbB2LJ (SD), synthetic peptides derived fromthe Leishmania NTPDase 1, and by serum samples from dogs with visceral leishmaniasis, identifying the antigenic L. infantum NTPDase 1 and, also, its conserved B domain (r83–122). By immunoprecipitation assays andWestern blots, immune sera SA and SB identified the catalytically active NTPDase 1 in promastigote preparation. In addition, the immune sera SB (44%) and SC or SD (87–99%) inhibited its activity, suggesting a direct effect on the B domain. By ELISA, 37%, 45% or 50% of 38 infected dogs were seropositive for r-pot B domain, LbB1LJ and LbB2LJ, respectively, confirming the B domain antigenicity.
URI: http://www.repositorio.ufop.br/handle/123456789/7263
metadata.dc.identifier.uri2: http://www.sciencedirect.com/science/article/pii/S1383576912001274
ISSN: 13835769
Appears in Collections:DEFAR - Artigos publicados em periódicos

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