Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/7246
Title: A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis.
Authors: Fraga, Deborah Bittencourt Mothé
Silva, Edimilson Domingos da
Pacheco, Luciano Vasconcellos
Borja, Lairton Souza
Oliveira, Isaac Queiroz de
Vital, Wendel Coura
Monteiro, Glória Regina
Oliveira, Geraldo Gileno de Sá
Jerônimo, Selma Maria Bezerra
Reis, Alexandre Barbosa
Veras, Patrícia Sampaio Tavares
Keywords: Serological diagnosis
Recombinant antigen
Rapid test
Multicentric study
Issue Date: 2014
Citation: FRAGA, D. B. M. et al. A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis. Parasites & Vectors, v. 7, p.136-139, 2014. Disponível em: <https://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-7-136>. Acesso em: 10 out. 2016.
Abstract: Background: Visceral leishmaniasis (VL) is a serious public health challenge in Brazil and dogs are considered to be the main urban reservoir of the causative agent. The culling of animals to control VL in some countries makes the accurate diagnosis of canine VL (CVL) essential. Recombinant antigens rLci1A and rLci2B were selected from a cDNA library of Leishmania infantum amastigotes due to their strong potential as candidates in diagnostic testing for CVL. The present multicentric study aimed to evaluate the sensitivity of a prototype test using these antigens (DPPrLci1A/rLci2B) against 154 sera obtained from symptomatic dogs within three endemic areas of VL in Brazil. The specificity was evaluated using 40 serum samples from negative dogs and dogs infected with other pathogens. Sensitivity and specificity rates of DPP rLci1A/rLci2B prototype were compared to rates from other diagnostic tests currently in use by the Brazilian Ministry of Health, including DPP®LVC, EIE®LVC. Findings: DPP rLci1A/rLci2B prototype offered similar performance to that offered by DPP®LVC rapid test, as follows: sensitivity of 87% (CI 81–91) and 88% (CI 82–93) and specificity of 100% (CI 91–100) and 97% (CI 87–100), respectively for DPP rLci1A/rLci2B and DPP®LVC. When results of these two tests were considered concomitantly, sensitivity increased to 93.5% (CI 89–96). Conclusions: The recombinant antigens rLci1A and rLci2B represent promising candidates for use in a multi-antigen rapid test for CVL. The inclusion of novel antigens to the DPP rLci1A/rLci2B prototype model could offer additionally enhanced sensitivity to detect animals infected by L. infantum.
URI: http://www.repositorio.ufop.br/handle/123456789/7246
ISSN: 17563305
metadata.dc.rights.license: Parasites & Vectors permite o arquivamento da versão PDF do editor. Fonte: Sherpa/Romeo <http://www.sherpa.ac.uk/romeo/issn/1756-3305/>. Acesso em: 03 jan. 2017.
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