Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/6629
Title: Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis.
Authors: Ferreira, Rafael Marini
Moreira, Leandro Marcio
Ferro, Jesus Aparecido
Silva, Marcia Regina Soares da
Laia, Marcelo Luiz de
Varani, Alessandro de Mello
Oliveira, Julio Cezar Franco de
Ferro, Maria Inês Tiraboschi
Keywords: Plant pathogen interaction
Medium inducing pathogenicity
Type III secretion system
Asymptomatic mutant
Virulence
Issue Date: 2016
Citation: FERREIRA, F. M. et al. Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis. PEERJ, v. 4, p. e1734, 2016. Disponível em: <https://peerj.com/articles/1734/#additional-information>. Acesso em: 16 jun. 2016.
Abstract: Citrus canker is a major disease affecting citrus production in Brazil. It's mainly caused by Xanthomonas citri subsp. citri strain 306 pathotype A (Xac). We analysed the differential expression of proteins secreted by wild type Xac and an asymptomatic mutant for hrpB4 (1hrpB4) grown in Nutrient Broth (NB) and a medium mimicking growth conditions in the plant (XAM1). This allowed the identification of 55 secreted proteins, of which 37 were secreted by both strains when cultured in XAM1. In this secreted protein repertoire, the following stand out: Virk, Polyphosphate-selective porin, Cellulase, Endoglucanase, Histone-like protein, Ribosomal proteins, five hypothetical proteins expressed only in the wild type strain, Lytic murein transglycosylase, Lipoprotein, Leucyl-tRNA synthetase, Co-chaperonin, Toluene tolerance, C-type cytochrome biogenesis membrane protein, Aminopeptidase and two hypothetical proteins expressed only in the1hrpB4 mutant. Furthermore, Peptidoglycan-associated outer membrane protein, Regulator of pathogenicity factor, Outer membrane proteins, Endopolygalacturonase, Chorismate mutase, Peptidyl-prolyl cis-trans isomerase and seven hypothetical proteins were detected in both strains, suggesting that there was no relationship with the secretion mediated by the type III secretory system, which is not functional in the mutant strain. Also worth mentioning is the Elongation factor Tu (EF-Tu), expressed only the wild type strain, and Type IV pilus assembly protein, Flagellin (FliC) and Flagellar hook-associated protein, identified in the wild-type strain secretome when grown only in NB. Noteworthy, that FliC, EF-Tu are classically characterized as PAMPs (Pathogen-associated molecular patterns), responsible for a PAMP-triggered immunity response. Therefore, our results highlight proteins potentially involved with the virulence. Overall, we conclude that the use of secretome data is a valuable approach that may bring more knowledge of the biology of this important plant pathogen, which ultimately can lead to the establishment of new strategies to combat citrus canker.
URI: http://www.repositorio.ufop.br/handle/123456789/6629
metadata.dc.identifier.doi: https://doi.org/10.7717/peerj.1734/supp-1
ISSN: 2167-9843 
metadata.dc.rights.license: Distributed under Creative Commons CC-BY 4.0 which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fonte: o próprio artigo.
Appears in Collections:DECBI - Artigos publicados em periódicos

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