Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/6584
Title: Clinical, hematological and biochemical alterations in hamster (Mesocricetus auratus) experimentally infected with Leishmania infantum through different routes of inoculation.
Authors: Moreira, Nádia das Dores
Souza, Juliana Vitoriano de
Roatt, Bruno Mendes
Vieira, Paula Melo de Abreu
Vital, Wendel Coura
Cardoso, Jamille Mirelle de Oliveira
Rezende, Mariana Trevisan
Ker, Henrique Gama
Giunchetti, Rodolfo Cordeiro
Carneiro, Cláudia Martins
Reis, Alexandre Barbosa
Keywords: Hamster
Mesocricetus auratus
Experimental infection
Hematological and biochemical alterations
Leishmania infantum
Issue Date: 2016
Citation: MOREIRA, N. das D. et al. Clinical, hematological and biochemical alterations in hamster (Mesocricetus auratus) experimentally infected with Leishmania infantum through different routes of inoculation. Parasites & Vectors, v. 9, p. 1-13, 2016. Disponível em: <http://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1464-y>. Acesso em: 16 jun. 2016.
Abstract: Background: Leishmaniasis remains among the most important parasitic diseases in the developing world and visceral leishmaniasis (VL) is the most fatal. The hamster Mesocricetus auratus is a susceptible model for the characterization of the disease, since infection of hamsters with L. infantum reproduces the clinical and pathological features of human VL. In this context, it provides a unique opportunity to study VL in its active form. The main goal of this study was to evaluate the clinical, biochemical, and hematological changes in male hamsters infected through different routes and strains of L. infantum. Methods: In the current study, hamsters (Mesocricetus auratus) were infected with the L. infantum strains (WHO/MHOM/BR/74/PP75 and MCAN/BR/2008/OP46) by intradermal, intraperitoneal and intracardiac routes. The animals were monitored for a nine month follow-up period. Results: The hamsters showed clinical signs similar to those observed in classical canine and human symptomatic VL, including splenomegaly, severe weight loss, anemia, and leucopenia. Therefore the OP46 strain was more infective, clinical signs were more frequent and more exacerbated in IC group with 80 to 100 % of the animals showing splenomegaly, in the last month infection. Additionally, desquamation, hair loss and external mucocutaneous lesions and ulcers localized in the snout, accompanied by swelling of the paws in all animals, were observed. Consequently, the animals presented severe weight loss/cachexia, hunched posture, an inability to eat or drink, and non-responsiveness to external stimuli. Furthermore, regardless of strain, route of inoculum and time assessed, the animals showed renal and hepatic alterations, with increased serum levels of urea and creatinine as well as elevated serum levels of aspartate aminotransferase and alanine aminotransferase. Conclusions: These results strongly suggest that the inoculation through the intracardiac route resulted in a higher severity among infections, especially in the sixth and ninth month after infection via intracardiac, exhibited clinical manifestations and biochemical/hematological findings similar to human visceral leishmaniasis. Therefore, we suggest that this route must be preferentially used in experimental infections for pathogenesis studies of VL in the hamster model.
metadata.dc.description.abstracten: https://doi.org/10.1186/s13071-016-1464-y
URI: http://www.repositorio.ufop.br/handle/123456789/6584
ISSN: 1756-3305
metadata.dc.rights.license: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Fonte: o próprio artigo.
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